4YVK
Crystal Structure of H. influenzae TrmD in complex with sinefungin and tRNA variant (G36C)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL32XU |
| Synchrotron site | SPring-8 |
| Beamline | BL32XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-02-19 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 87.450, 87.450, 228.720 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.725 - 3.002 |
| R-factor | 0.2218 |
| Rwork | 0.218 |
| R-free | 0.28320 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4yvi |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.334 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.7_650)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 3.000 |
| Number of reflections | 18509 |
| <I/σ(I)> | 20.2 |
| Completeness [%] | 99.8 |
| Redundancy | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | sodium acetate trihydrate, ammonium phosphate monobasic, PEG 20000 |
