4YTQ

Crystal structure of D-tagatose 3-epimerase C66S from Pseudomonas cichorii in complex with 1-deoxy D-tagatose

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	D-tagatose 3-epimerase	polymer	300	33858.6	4	UniProt (O50580) Pfam (PF01261)	Pseudomonas cichorii
2	E, G, H, J (A, B, C, D)	MANGANESE (II) ION	non-polymer		54.9	4	Chemie (MN)
3	F (A)	1-deoxy-D-tagatose	non-polymer		164.2	1	Chemie (TGD)
4	I (C)	1-deoxy-alpha-D-tagatopyranose	non-polymer		164.2	1	Chemie (TGY)
5	K, L, M, N (A, B, C, D)	water	water		18.0	1229	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 290 (UniProt: O50580)

PDB	External Database	Details
Ser 66	Cys 66	engineered mutation
Gly 291	-	expression tag
Ser 292	-	expression tag
Arg 293	-	expression tag
Ser 294	-	expression tag
His 295	-	expression tag
His 296	-	expression tag
His 297	-	expression tag
His 298	-	expression tag
His 299	-	expression tag
His 300	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	135434.5
Non-Polymers*	Number of molecules	6
	Total formula weight	548.1
All*	Total formula weight	135982.5

*Water molecules are not included.