4YP3
Mutant Human DNA Polymerase Eta Q38A/R61A Inserting dCTP Opposite an 8-Oxoguanine Lesion
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | DNA polymerase eta | polymer | 435 | 48474.5 | 1 | UniProt (Q9Y253) Pfam (PF00817) Pfam (PF21704) Pfam (PF11799) | Homo sapiens (Human) | RAD30 homolog A,Xeroderma pigmentosum variant type protein |
| 2 | B (T) | DNA (5'-D(*TP*(8OG)P*AP*TP*GP*AP*CP*GP*CP*T)-3') | polymer | 12 | 3678.4 | 1 | synthetic construct | ||
| 3 | C (P) | DNA (5'-D(*AP*GP*CP*GP*TP*CP*AP*T)-3') | polymer | 8 | 2426.6 | 1 | synthetic construct | ||
| 4 | D (A) | 2'-DEOXYCYTIDINE-5'-TRIPHOSPHATE | non-polymer | 467.2 | 1 | Chemie (DCP) | |||
| 5 | E, F (A) | CALCIUM ION | non-polymer | 40.1 | 2 | Chemie (CA) | |||
| 6 | G, H (A) | GLYCEROL | non-polymer | 92.1 | 2 | Chemie (GOL) | |||
| 7 | I, J, K (A, T, P) | water | water | 18.0 | 384 | Chemie (HOH) |
Sequence modifications
A: 1 - 432 (UniProt: Q9Y253)
| PDB | External Database | Details |
|---|---|---|
| Gly -2 | - | expression tag |
| Pro -1 | - | expression tag |
| His 0 | - | expression tag |
| Ala 38 | Gln 38 | engineered mutation |
| Ala 61 | Arg 61 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 3 |
| Total formula weight | 54579.6 | |
| Non-Polymers* | Number of molecules | 5 |
| Total formula weight | 731.5 | |
| All* | Total formula weight | 55311.1 |
