4YLO

E. coli Transcription Initiation Complex - 16-bp spacer and 4-nt RNA

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, G, H, M...	DNA-directed RNA polymerase subunit alpha	polymer	242	26899.6	6	UniProt (A7ZSI4) Pfam (PF01193) Pfam (PF01000) In PDB	Escherichia coli	RNAP subunit alpha, RNA polymerase subunit alpha, Transcriptase subunit alpha
2	C, I, O	DNA-directed RNA polymerase subunit beta	polymer	1342	150820.9	3	UniProt (A7ZUK1) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562) Pfam (PF04560) In PDB	Escherichia coli	RNAP subunit beta, RNA polymerase subunit beta, Transcriptase subunit beta
3	D, J, P	DNA-directed RNA polymerase subunit beta'	polymer	1407	155366.8	3	UniProt (A7ZUK2) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) UniProt (by SIFTS) (P0A8T7) In PDB	Escherichia coli	RNAP subunit beta', RNA polymerase subunit beta', Transcriptase subunit beta'
4	E, K, Q	DNA-directed RNA polymerase subunit omega	polymer	90	10118.4	3	UniProt (A7ZTK1) Pfam (PF01192) In PDB	Escherichia coli	RNAP omega subunit, RNA polymerase omega subunit, Transcriptase subunit omega
5	F, L, R	RNA polymerase sigma factor RpoD	polymer	628	72206.3	3	UniProt (P00579) Pfam (PF03979) Pfam (PF00140) Pfam (PF04546) Pfam (PF04542) Pfam (PF04539) Pfam (PF04545) In PDB	Escherichia coli	Sigma-70
6	1, 4, 7	NT strand DNA (49-MER)	polymer	49	15024.6	3		synthetic construct
7	2, 5, 8	T strand DNA (49-MER)	polymer	49	15233.8	3		synthetic construct
8	3, 6, 9	RNA (5'-D(*(GTP))-R(P*AP*GP*U)-3')	polymer	4	1440.8	3		synthetic construct
9	C, J, P	MAGNESIUM ION	non-polymer		24.3	3	Chemie (MG)
10	D, J, P	ZINC ION	non-polymer		65.4	6	Chemie (ZN)

Sequence modifications

A, B, G, H, M, N: 1 - 235 (UniProt: A7ZSI4)

PDB	External Database	Details
Ala -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag

F, L, R: 1 - 613 (UniProt: P00579)

PDB	External Database	Details
Met -14	-	expression tag
Arg -13	-	expression tag
Gly -12	-	expression tag
Ser -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
Thr -4	-	expression tag
Asp -3	-	expression tag
Gln -2	-	expression tag
Phe -1	-	expression tag
Thr 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	27
	Total formula weight	1422031.8
Non-Polymers*	Number of molecules	9
	Total formula weight	465.4
All*	Total formula weight	1422497.2

*Water molecules are not included.