4YLO
E. coli Transcription Initiation Complex - 16-bp spacer and 4-nt RNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-09-21 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.1 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 240.888, 208.174, 256.323 |
Unit cell angles | 90.00, 119.31, 90.00 |
Refinement procedure
Resolution | 39.950 - 6.000 |
R-factor | 0.23151 |
Rwork | 0.227 |
R-free | 0.31429 |
RMSD bond length | 0.012 |
RMSD bond angle | 1.611 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 40.000 |
High resolution limit [Å] | 6.000 |
Number of reflections | 55356 |
<I/σ(I)> | 13.4 |
Completeness [%] | 99.9 |
Redundancy | 5.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 7% PEG3350, 150 mM magnesium chloride, 100 mM HEPES sodium, pH 7.0 |