4YIZ

Crystal structure of engineered TgAMA1 lacking the DII loop in complex with an Eimeria tenella RON2D3 peptide

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C, E	Apical membrane antigen AMA1	polymer	419	46064.3	3	UniProt (S8GKS3) Pfam (PF02430) In PDB	Toxoplasma gondii ME49
2	B, D, F	Rhoptry neck protein 2, putative	polymer	40	3872.4	3	UniProt (U6KQJ2) In PDB	Eimeria tenella (Coccidian parasite)
3	A, C, E	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	3	Chemie (NAG)
4	A	2-acetamido-2-deoxy-alpha-D-galactopyranose	non-polymer		221.2	1	Chemie (A2G)
5	A, C, E	SULFATE ION	non-polymer		96.1	5	Chemie (SO4)
6		water	water		18.0	487	Chemie (HOH)

Sequence modifications

A, C, E: 64 - 484 (UniProt: S8GKS3)

PDB	External Database	Details
Gly 59	-	expression tag
Ser 60	-	expression tag
Ala 61	-	expression tag
Met 62	-	expression tag
Gly 63	-	expression tag
-	His 340	deletion
-	Thr 341	deletion
-	Tyr 342	deletion
-	Pro 343	deletion
-	Leu 344	deletion
-	Thr 345	deletion
-	Ser 346	deletion
-	Gln 347	deletion
-	Ala 348	deletion
-	Ser 349	deletion
-	Trp 350	deletion
-	Asn 351	deletion
-	Asp 352	deletion
Gly 353	Trp 353	linker
Ser 354	Trp 354	linker
Gly 355	Pro 355	linker
Ser 356	Leu 356	linker
Gly 357	His 357	linker
Ser 358	Gln 358	linker
Gly 359	Ser 359	linker
Ala 485	-	expression tag
Ala 486	-	expression tag
Leu 487	-	expression tag
Val 488	-	expression tag
Pro 489	-	expression tag
Arg 490	-	expression tag

B, D, F: 1261 - 1297 (UniProt: U6KQJ2)

PDB	External Database	Details
Gly 1258	-	expression tag
Ser 1259	-	expression tag
Ala 1260	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	149810.0
Non-Polymers*	Number of molecules	9
	Total formula weight	1365.1
All*	Total formula weight	151175.1

*Water molecules are not included.