4YIZ
Crystal structure of engineered TgAMA1 lacking the DII loop in complex with an Eimeria tenella RON2D3 peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-04-18 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 265.928, 265.928, 94.160 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 59.500 - 2.200 |
| R-factor | 0.188 |
| Rwork | 0.187 |
| R-free | 0.20700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ID 2Y8T |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.845 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 72.890 | 2.320 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.133 | 0.475 |
| Number of reflections | 180412 | |
| <I/σ(I)> | 6.6 | 3.4 |
| Completeness [%] | 93.9 | 94.4 |
| Redundancy | 4.3 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 291 | 0.1 M tri-sodium citrate pH 5.0 and 2.0 M ammonium sulfate |
