4YHX
Crystal Structure of LAGLIDADG Meganuclease I-GpeMI Bound to Uncleaved DNA
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Ribosomal protein 3/homing endonuclease-like fusion protein | polymer | 308 | 34857.1 | 1 | UniProt (C7SQG1) Pfam (PF00961) | Grosmannia penicillata | |
| 2 | B (B) | DNA (27-MER) | polymer | 27 | 8049.2 | 1 | synthetic construct | ||
| 3 | C (C) | DNA (27-MER) | polymer | 27 | 8543.5 | 1 | synthetic construct | ||
| 4 | D, E, F (A) | CALCIUM ION | non-polymer | 40.1 | 3 | Chemie (CA) | |||
| 5 | G, H, I (A, B, C) | water | water | 18.0 | 219 | Chemie (HOH) |
Sequence modifications
A: 1 - 308 (UniProt: C7SQG1)
| PDB | External Database | Details |
|---|---|---|
| Lys 51 | Ile 470 | engineered mutation |
| Glu 215 | Ala 634 | engineered mutation |
| Glu 260 | Val 679 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 3 |
| Total formula weight | 51449.8 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 120.2 | |
| All* | Total formula weight | 51570.0 |
