4YHX
Crystal Structure of LAGLIDADG Meganuclease I-GpeMI Bound to Uncleaved DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-07-13 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 151.985, 39.895, 73.986 |
| Unit cell angles | 90.00, 90.43, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.150 |
| R-factor | 0.1812 |
| Rwork | 0.178 |
| R-free | 0.24400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3qqy |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.627 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0071) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.230 |
| High resolution limit [Å] | 2.150 | 4.630 | 2.150 |
| Rmerge | 0.101 | 0.057 | 0.455 |
| Rmeas | 0.119 | 0.068 | 0.547 |
| Rpim | 0.063 | 0.036 | 0.301 |
| Total number of observations | 84408 | ||
| Number of reflections | 24373 | ||
| <I/σ(I)> | 14.8 | ||
| Completeness [%] | 99.3 | 97.8 | 97.6 |
| Redundancy | 3.5 | 3.3 | 3 |
| CC(1/2) | 0.991 | 0.794 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 22.5% PEG 3000, 50mM CaCl2 |
