4YGX

Crystal Structure of D. melanogaster Ssu72+Symplekin bound to cis peptidomimetic CTD phospho-Ser5 peptide

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (A, C)	Symplekin	polymer	339	37495.3	2	UniProt (Q8MSU4) Pfam (PF11935)	Drosophila melanogaster (Fruit fly)
2	B, D (B, D)	LD40846p	polymer	200	23130.3	2	UniProt (Q9VWE4) Pfam (PF04722)	Drosophila melanogaster (Fruit fly)	Ssu72 ortholog,isoform A,isoform C
3	E (E)	cis peptidomimetic CTD phospho-Ser5 peptide	polymer	12	1259.2	1		synthetic construct
4	F, G, H, I (A, B, C, D)	water	water		18.0	10	Chemie (HOH)

Sequence modifications

A, C: 19 - 351 (UniProt: Q8MSU4)

PDB	External Database	Details
Gly 13	-	expression tag
Pro 14	-	expression tag
Gly 15	-	expression tag
Ser 16	-	expression tag
Gly 17	-	expression tag
Met 18	-	expression tag

B, D: 1 - 195 (UniProt: Q9VWE4)

PDB	External Database	Details
Gly -4	-	expression tag
Pro -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Gly 0	-	expression tag
Asp 13	Cys 13	engineered mutation
Asn 144	Asp 144	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	122510.3
All*	Total formula weight	122510.3

*Water molecules are not included.