4YGE

Crystal structure of ERGIC-53/MCFD2, trigonal calcium-bound form 2

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C, E (A, C, E)	Protein ERGIC-53	polymer	246	27154.2	3	UniProt (P49257) Pfam (PF03388)	Homo sapiens (Human)	ER-Golgi intermediate compartment 53 kDa protein,Gp58,Intracellular mannose-specific lectin MR60,Lectin mannose-binding 1
2	B, D, F (B, D, F)	Multiple coagulation factor deficiency protein 2	polymer	143	16316.8	3	UniProt (Q8NI22) Pfam (PF13499)	Homo sapiens (Human)	Neural stem cell-derived neuronal survival protein
3	G, H, J, K, L... (A, B, C, D, F)	CALCIUM ION	non-polymer		40.1	9	Chemie (CA)
4	I, M, P (A, C, E)	CHLORIDE ION	non-polymer		35.5	3	Chemie (CL)
5	S, T, U, V (A, C, E, F)	water	water		18.0	10	Chemie (HOH)

Sequence modifications

A, C, E: 31 - 269 (UniProt: P49257)

PDB	External Database	Details
Met 24	-	expression tag
Asn 25	-	expression tag
His 26	-	expression tag
Lys 27	-	expression tag
Val 28	-	expression tag
His 29	-	expression tag
Met 30	-	expression tag

B, D, F: 27 - 146 (UniProt: Q8NI22)

PDB	External Database	Details
Met 4	-	expression tag
Gly 5	-	expression tag
His 6	-	expression tag
His 7	-	expression tag
His 8	-	expression tag
His 9	-	expression tag
His 10	-	expression tag
His 11	-	expression tag
His 12	-	expression tag
His 13	-	expression tag
His 14	-	expression tag
His 15	-	expression tag
Ser 16	-	expression tag
Ser 17	-	expression tag
Gly 18	-	expression tag
His 19	-	expression tag
Ile 20	-	expression tag
Glu 21	-	expression tag
Gly 22	-	expression tag
Arg 23	-	expression tag
His 24	-	expression tag
Met 25	-	expression tag
Leu 26	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	130413.2
Non-Polymers*	Number of molecules	12
	Total formula weight	467.1
All*	Total formula weight	130880.3

*Water molecules are not included.