4YFU

Crystal structure of open Bacillus fragment DNA polymerase bound to DNA and dTTP

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, D	DNA polymerase	polymer	580	66279.1	2	UniProt (E1C9K5) Pfam (PF22619) Pfam (PF00476) In PDB	Geobacillus stearothermophilus
2	B, E	Primer DNA	polymer	9	2714.8	2		synthetic construct
3	C, F	Template DNA	polymer	14	4329.8	2		synthetic construct
4	G, H	beta-D-fructofuranose-(2-1)-alpha-D-glucopyranose	branched		342.3	2	In PDB BIRD (PRD_900003)		sucrose
5	A	THYMIDINE-5'-TRIPHOSPHATE	non-polymer		482.2	1	Chemie (TTP)
6	D	SULFATE ION	non-polymer		96.1	1	Chemie (SO4)
7		water	water		18.0	1371	Chemie (HOH)

Sequence modifications

A, D: 298 - 876 (UniProt: E1C9K5)

PDB	External Database	Details
Lys 297	-	expression tag
Glu 456	Ala 160	conflict
Lys 505	Glu 209	conflict
Gly 512	Arg 216	conflict
Thr 550	Ser 254	conflict
Ala 598	Asp 302	engineered mutation
Tyr 710	Phe 414	engineered mutation
His 823	Arg 527	conflict

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	146647.4
Branched	Number of molecules	2
	Total formula weight	684.6
Non-Polymers*	Number of molecules	2
	Total formula weight	578.2
All*	Total formula weight	147910.2

*Water molecules are not included.