4YFU
Crystal structure of open Bacillus fragment DNA polymerase bound to DNA and dTTP
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, D) | DNA polymerase | polymer | 580 | 66279.1 | 2 | UniProt (E1C9K5) Pfam (PF22619) Pfam (PF00476) | Geobacillus stearothermophilus | |
| 2 | C, E (B, E) | Primer DNA | polymer | 9 | 2714.8 | 2 | synthetic construct | ||
| 3 | D, F (C, F) | Template DNA | polymer | 14 | 4329.8 | 2 | synthetic construct | ||
| 4 | G, H (G, H) | beta-D-fructofuranose-(2-1)-alpha-D-glucopyranose | branched | 342.3 | 2 | In PDB BIRD (PRD_900003) | sucrose | ||
| 5 | I (A) | THYMIDINE-5'-TRIPHOSPHATE | non-polymer | 482.2 | 1 | Chemie (TTP) | |||
| 6 | J (D) | SULFATE ION | non-polymer | 96.1 | 1 | Chemie (SO4) | |||
| 7 | K, L, M, N, O... (A, D, B, C, E...) | water | water | 18.0 | 1371 | Chemie (HOH) |
Sequence modifications
A, D: 298 - 876 (UniProt: E1C9K5)
| PDB | External Database | Details |
|---|---|---|
| Lys 297 | - | expression tag |
| Glu 456 | Ala 160 | conflict |
| Lys 505 | Glu 209 | conflict |
| Gly 512 | Arg 216 | conflict |
| Thr 550 | Ser 254 | conflict |
| Ala 598 | Asp 302 | engineered mutation |
| Tyr 710 | Phe 414 | engineered mutation |
| His 823 | Arg 527 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 6 |
| Total formula weight | 146647.4 | |
| Branched | Number of molecules | 2 |
| Total formula weight | 684.6 | |
| Non-Polymers* | Number of molecules | 2 |
| Total formula weight | 578.2 | |
| All* | Total formula weight | 147910.2 |
