4YFU

Crystal structure of open Bacillus fragment DNA polymerase bound to DNA and dTTP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2007-06-23
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	93.230, 108.100, 151.340
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.680 - 1.500
R-factor	0.1966
Rwork	0.195
R-free	0.23030
RMSD bond length	0.024
RMSD bond angle	2.529
Data reduction software	DENZO
Data scaling software	XSCALE
Phasing software	REFMAC
Refinement software	REFMAC (5.8.0103)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	44.700		1.590
High resolution limit [Å]	1.500	4.470	1.500
Rmerge	0.063	0.050	0.498
Rmeas	0.071	0.058	0.584
Total number of observations	1111808
Number of reflections	241163	9170	37259
<I/σ(I)>	13.33	26.56	2.79
Completeness [%]	99.0	94.4	96.2
Redundancy	4.61		3.38

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.8	290	~50% ammonium sulfate, MES pH 5.8, magnesium sulfate, methyl pentanediol