4YD1

Ternary complex of human DNA Polymerase Mu with 2-nt gapped DNA substrate and an incoming nonhydrolyzable dUMPNPP

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	DNA-directed DNA/RNA polymerase mu	polymer	354	39844.2	1	UniProt (Q9NP87) Pfam (PF14716) Pfam (PF10391) Pfam (PF14792) Pfam (PF14791) In PDB	Homo sapiens (Human)	Pol Mu,Terminal transferase
2	T	DNA (5'-D(*CP*GP*GP*CP*AP*AP*TP*AP*CP*G)-3')	polymer	10	3054.0	1		Homo sapiens
3	P	DNA (5'-D(*CP*GP*TP*A)-3')	polymer	4	1190.8	1		Homo sapiens
4	D	DNA (5'-D(P*GP*CP*CP*G)-3')	polymer	4	1191.8	1		Homo sapiens
5	A, T	MAGNESIUM ION	non-polymer		24.3	3	Chemie (MG)
6	A	1,2-ETHANEDIOL	non-polymer		62.1	2	Chemie (EDO)
7	A	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
8	A	2'-DEOXYURIDINE 5'-ALPHA,BETA-IMIDO-TRIPHOSPHATE	non-polymer		467.2	1	Chemie (DUP)
9	A	CHLORIDE ION	non-polymer		35.5	4	Chemie (CL)
10	A	SODIUM ION	non-polymer		23.0	1	Chemie (NA)
11		water	water		18.0	468	Chemie (HOH)

Sequence modifications

A: 134 - 494 (UniProt: Q9NP87)

PDB	External Database	Details
Gly 129	-	expression tag
Ser 130	-	expression tag
Ala 131	-	expression tag
Ala 132	-	expression tag
Ala 133	-	expression tag
-	Pro 398	deletion
-	Gly 399	deletion
-	Ala 400	deletion
-	Ala 401	deletion
-	Val 402	deletion
-	Gly 403	deletion
-	Gly 404	deletion
-	Ser 405	deletion
-	Thr 406	deletion
-	Arg 407	deletion
-	Pro 408	deletion
-	Cys 409	deletion
Gly 410	Pro 410	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	45280.8
Non-Polymers*	Number of molecules	13
	Total formula weight	1013.2
All*	Total formula weight	46294.0

*Water molecules are not included.