4YCO

E. coli dihydrouridine synthase C (DusC) in complex with tRNAPhe

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C (A, B, C)	tRNA-dihydrouridine synthase C	polymer	321	35780.0	3	UniProt (P33371) Pfam (PF01207)	Escherichia coli (strain K12)
2	D, E, F (D, E, F)	tRNAphe	polymer	76	24485.5	3	GenBank (731469900)	Escherichia coli K-12
3	G, I, J (A, B, C)	FLAVIN MONONUCLEOTIDE	non-polymer		456.3	3	Chemie (FMN)
4	AA, BA, DA, EA, FA... (E, F, B, D)	MAGNESIUM ION	non-polymer		24.3	25	Chemie (MG)
5	CA, K, T (F, D, E)	MANGANESE (II) ION	non-polymer		54.9	3	Chemie (MN)
6	LA, MA, NA, OA, PA... (A, B, C, D, E...)	water	water		18.0	614	Chemie (HOH)

Sequence modifications

A, B, C: 1 - 315 (UniProt: P33371)

PDB	External Database	Details
Gly -5	-	expression tag
Ser -4	-	expression tag
His -3	-	expression tag
Met -2	-	expression tag
Ala -1	-	expression tag
Ser 0	-	expression tag
Ala 98	Cys 98	engineered mutation

D, E, F: 1 - 76 (GenBank: 731469900)

PDB	External Database	Details
G 3	C 3094461	engineered mutation
C 70	G 3094528	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	180796.6
Non-Polymers*	Number of molecules	31
	Total formula weight	2141.5
All*	Total formula weight	182938.1

*Water molecules are not included.