Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

4YCO

E. coli dihydrouridine synthase C (DusC) in complex with tRNAPhe

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000049	molecular_function	tRNA binding
A	0002943	biological_process	tRNA dihydrouridine synthesis
A	0003723	molecular_function	RNA binding
A	0008033	biological_process	tRNA processing
A	0010181	molecular_function	FMN binding
A	0010467	biological_process	gene expression
A	0016491	molecular_function	oxidoreductase activity
A	0017150	molecular_function	tRNA dihydrouridine synthase activity
A	0050660	molecular_function	flavin adenine dinucleotide binding
A	0102262	molecular_function	tRNA-dihydrouridine16 synthase activity
B	0000049	molecular_function	tRNA binding
B	0002943	biological_process	tRNA dihydrouridine synthesis
B	0003723	molecular_function	RNA binding
B	0008033	biological_process	tRNA processing
B	0010181	molecular_function	FMN binding
B	0010467	biological_process	gene expression
B	0016491	molecular_function	oxidoreductase activity
B	0017150	molecular_function	tRNA dihydrouridine synthase activity
B	0050660	molecular_function	flavin adenine dinucleotide binding
B	0102262	molecular_function	tRNA-dihydrouridine16 synthase activity
C	0000049	molecular_function	tRNA binding
C	0002943	biological_process	tRNA dihydrouridine synthesis
C	0003723	molecular_function	RNA binding
C	0008033	biological_process	tRNA processing
C	0010181	molecular_function	FMN binding
C	0010467	biological_process	gene expression
C	0016491	molecular_function	oxidoreductase activity
C	0017150	molecular_function	tRNA dihydrouridine synthase activity
C	0050660	molecular_function	flavin adenine dinucleotide binding
C	0102262	molecular_function	tRNA-dihydrouridine16 synthase activity

Functional Information from PDB Data

site_id	AC1
Number of Residues	19
Details	binding site for residue FMN A 401

Chain	Residue
A	ALA6
A	TYR176
A	ASN200
A	GLY201
A	GLU202
A	GLY224
A	ARG225
A	HOH511
A	HOH513
A	HOH551
D	U16
A	PRO7
A	MET8
A	GLU9
A	PHE33
A	GLN68
A	ASN95
A	LYS139
A	HIS168

site_id	AC2
Number of Residues	4
Details	binding site for residue MG B 401

Chain	Residue
B	HOH513
B	HOH514
E	HOH212
E	HOH213

site_id	AC3
Number of Residues	19
Details	binding site for residue FMN B 402

Chain	Residue
B	ALA6
B	PRO7
B	MET8
B	GLU9
B	PHE33
B	GLN68
B	ASN95
B	LYS139
B	HIS168
B	TYR176
B	ASN200
B	GLY201
B	GLU202
B	GLY224
B	ARG225
B	HOH526
B	HOH543
B	HOH578
E	U16

site_id	AC4
Number of Residues	19
Details	binding site for residue FMN C 401

Chain	Residue
C	ALA6
C	PRO7
C	MET8
C	GLU9
C	PHE33
C	GLN68
C	ASN95
C	LYS139
C	HIS168
C	TYR176
C	ASN200
C	GLY201
C	GLU202
C	GLY224
C	ARG225
C	HOH513
C	HOH566
C	HOH567
F	U16

site_id	AC5
Number of Residues	6
Details	binding site for residue MN D 101

Chain	Residue
D	G15
D	HOH249
D	HOH250
D	HOH251
D	HOH253
D	HOH254

site_id	AC6
Number of Residues	6
Details	binding site for residue MG D 102

Chain	Residue
D	HOH215
D	HOH216
D	HOH217
D	HOH218
D	HOH219
D	HOH220

site_id	AC7
Number of Residues	6
Details	binding site for residue MG D 103

Chain	Residue
D	U51
D	HOH221
D	HOH222
D	HOH223
D	HOH224
D	HOH225

site_id	AC8
Number of Residues	6
Details	binding site for residue MG D 104

Chain	Residue
D	HOH226
D	HOH227
D	HOH228
D	HOH229
D	HOH230
D	HOH231

site_id	AC9
Number of Residues	6
Details	binding site for residue MG D 105

Chain	Residue
D	HOH232
D	HOH233
D	HOH234
D	HOH235
D	HOH236
D	HOH237

site_id	AD1
Number of Residues	6
Details	binding site for residue MG D 106

Chain	Residue
D	G18
D	HOH201
D	HOH212
D	HOH214
D	HOH238
D	HOH239

site_id	AD2
Number of Residues	6
Details	binding site for residue MG D 107

Chain	Residue
D	G19
D	HOH240
D	HOH241
D	HOH242
A	HOH501
D	C17

site_id	AD3
Number of Residues	5
Details	binding site for residue MG D 108

Chain	Residue
D	HOH211
E	G34
E	HOH201
E	HOH202
E	HOH204

site_id	AD4
Number of Residues	3
Details	binding site for residue MG D 109

Chain	Residue
D	U45
D	G46
D	HOH243

site_id	AD5
Number of Residues	5
Details	binding site for residue MN E 101

Chain	Residue
E	G15
E	HOH246
E	HOH247
E	HOH256
E	HOH257

site_id	AD6
Number of Residues	6
Details	binding site for residue MG E 102

Chain	Residue
E	HOH206
E	HOH207
E	HOH208
E	HOH209
E	HOH210
E	HOH211

site_id	AD7
Number of Residues	5
Details	binding site for residue MG E 103

Chain	Residue
E	G18
E	HOH214
E	HOH215
E	HOH216
E	HOH217

site_id	AD8
Number of Residues	6
Details	binding site for residue MG E 104

Chain	Residue
E	G22
E	G44
E	U45
E	G46
E	HOH218
E	HOH219

site_id	AD9
Number of Residues	6
Details	binding site for residue MG E 105

Chain	Residue
E	HOH220
E	HOH221
E	HOH222
E	HOH223
E	HOH224
E	HOH225

site_id	AE1
Number of Residues	6
Details	binding site for residue MG E 106

Chain	Residue
B	HOH515
E	C17
E	G19
E	HOH226
E	HOH227
E	HOH228

site_id	AE2
Number of Residues	6
Details	binding site for residue MG E 107

Chain	Residue
E	U51
E	HOH229
E	HOH230
E	HOH231
E	HOH232
E	HOH233

site_id	AE3
Number of Residues	7
Details	binding site for residue MG E 108

Chain	Residue
E	U8
E	U12
E	HOH234
E	HOH235
E	HOH236
E	HOH237
E	HOH238

site_id	AE4
Number of Residues	6
Details	binding site for residue MG E 109

Chain	Residue
E	HOH239
E	HOH240
E	HOH241
E	HOH242
E	HOH243
E	HOH244

site_id	AE5
Number of Residues	6
Details	binding site for residue MN F 101

Chain	Residue
F	G15
F	HOH253
F	HOH254
F	HOH255
F	HOH256
F	HOH292

site_id	AE6
Number of Residues	6
Details	binding site for residue MG F 102

Chain	Residue
F	HOH208
F	HOH209
F	HOH210
F	HOH211
F	HOH212
F	HOH213

site_id	AE7
Number of Residues	6
Details	binding site for residue MG F 103

Chain	Residue
F	HOH214
F	HOH215
F	HOH216
F	HOH217
F	HOH218
F	HOH219

site_id	AE8
Number of Residues	6
Details	binding site for residue MG F 104

Chain	Residue
F	U51
F	HOH220
F	HOH221
F	HOH222
F	HOH223
F	HOH224

site_id	AE9
Number of Residues	6
Details	binding site for residue MG F 105

Chain	Residue
D	HOH213
F	G18
F	HOH201
F	HOH207
F	HOH225
F	HOH226

site_id	AF1
Number of Residues	6
Details	binding site for residue MG F 106

Chain	Residue
F	U45
F	G46
F	HOH227
F	HOH228
F	HOH229
F	HOH230

site_id	AF2
Number of Residues	6
Details	binding site for residue MG F 107

Chain	Residue
F	U12
F	HOH231
F	HOH232
F	HOH233
F	HOH234
F	HOH235

site_id	AF3
Number of Residues	5
Details	binding site for residue MG F 108

Chain	Residue
F	HOH236
F	HOH237
F	HOH238
F	HOH239
F	HOH240

site_id	AF4
Number of Residues	6
Details	binding site for residue MG F 109

Chain	Residue
C	HOH511
F	C17
F	G19
F	HOH241
F	HOH242
F	HOH243

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	3
Details	ACT_SITE: Proton donor => ECO:0000250\|UniProtKB:Q5SMC7, ECO:0000305\|PubMed:25902496

Chain	Residue	Details
A	ALA98
B	ALA98
C	ALA98

site_id	SWS_FT_FI2
Number of Residues	15
Details	BINDING: BINDING => ECO:0000269\|PubMed:23908023, ECO:0000269\|PubMed:25902496, ECO:0007744\|PDB:3W9Z, ECO:0007744\|PDB:4BF9, ECO:0007744\|PDB:4BFA

Chain	Residue	Details
A	PRO7
B	GLY224
C	PRO7
C	GLN68
C	LYS139
C	ASN200
C	GLY224
A	GLN68
A	LYS139
A	ASN200
A	GLY224
B	PRO7
B	GLN68
B	LYS139
B	ASN200

site_id	SWS_FT_FI3
Number of Residues	12
Details	SITE: Interacts with tRNA; defines subfamily-specific binding signature => ECO:0000269\|PubMed:25902496

Chain	Residue	Details
A	ARG35
C	ARG272
C	LYS274
C	ARG295
A	ARG272
A	LYS274
A	ARG295
B	ARG35
B	ARG272
B	LYS274
B	ARG295
C	ARG35

site_id	SWS_FT_FI4
Number of Residues	9
Details	SITE: Interacts with tRNA => ECO:0000269\|PubMed:25902496

Chain	Residue	Details
A	ASN95
A	TYR176
A	TYR279
B	ASN95
B	TYR176
B	TYR279
C	ASN95
C	TYR176
C	TYR279

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)