4Y5V

Diabody 305 complex with EpoR

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, D, G (A, D, G)	diabody 305 VH domain	polymer	130	13819.2	3		Homo sapiens
2	B, E, H (B, E, H)	Diabody 305 VL domain	polymer	117	12079.2	3		Homo sapiens
3	C, F, I (C, F, I)	Erythropoietin receptor	polymer	229	25129.4	3	UniProt (P19235) Pfam (PF09067) Pfam (PF00041)	Homo sapiens (Human)	EPO-R
4	J, K, L, M, N... (A, B, D, E, F...)	GLYCEROL	non-polymer		92.1	15	Chemie (GOL)
5	P (D)	TRIETHYLENE GLYCOL	non-polymer		150.2	1	Chemie (PGE)
6	Q (D)	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	1	Chemie (PEG)
7	AA, BA, CA, DA, EA... (A, B, C, D, E...)	water	water		18.0	301	Chemie (HOH)

Sequence modifications

C, F, I: 8 - 225 (UniProt: P19235)

PDB	External Database	Details
Phe 3	-	expression tag
Ala 4	-	expression tag
Gly 5	-	expression tag
Ser 6	-	expression tag
Ala 7	-	expression tag
Gln 52	Asn 76	engineered mutation
Gln 164	Asn 188	engineered mutation
Lys 226	-	expression tag
Glu 227	-	expression tag
Lys 228	-	expression tag
Ala 229	-	expression tag
Ala 230	-	expression tag
Ala 231	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	9
	Total formula weight	153083.6
Non-Polymers*	Number of molecules	17
	Total formula weight	1637.7
All*	Total formula weight	154721.3

*Water molecules are not included.