4XTC

Crystal structure of bacterial alginate ABC transporter in complex with alginate pentasaccharide-bound periplasmic protein

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (M)	AlgM1	polymer	301	34286.6	1	UniProt (Q9KWT8)	Sphingomonas sp. A1
2	B (N)	AlgM2	polymer	305	34496.6	1	UniProt (Q9KWT7)	Sphingomonas sp. A1
3	C, E (S, T)	AlgS	polymer	363	39574.5	2	UniProt (Q9KWT9)	Sphingomonas sp. A1
4	D (Q)	AlgQ2	polymer	516	59688.8	1	UniProt (Q9KWT5)	Sphingomonas sp. A1
5	F (A)	beta-D-mannopyranuronic acid-(1-4)-beta-D-mannopyranuronic acid-(1-4)-beta-D-mannopyranuronic acid-(1-4)-beta-D-mannopyranuronic acid-(1-4)-beta-D-mannopyranuronic acid	branched		898.6	1	In PDB GlyTouCan (G61688UV)

Sequence modifications

M: 1 - 324 (UniProt: Q9KWT8)

PDB	External Database	Details
-	Thr 2	deletion
-	Ser 3	deletion
-	Ala 4	deletion
-	Thr 5	deletion
-	Lys 6	deletion
-	Ala 7	deletion
-	Gln 8	deletion
-	Ser 9	deletion
-	Ile 10	deletion
-	Pro 11	deletion
-	Leu 12	deletion
-	Pro 13	deletion
-	Ala 14	deletion
-	Ala 15	deletion
-	Thr 16	deletion
-	Leu 17	deletion
-	Asp 18	deletion
-	Val 19	deletion
-	Arg 20	deletion
-	Ser 21	deletion
-	Lys 22	deletion
-	Pro 23	deletion
-	Leu 24	deletion

N: 1 - 293 (UniProt: Q9KWT7)

PDB	External Database	Details
Leu 294	-	expression tag
Glu 295	-	expression tag
His 296	-	expression tag
His 297	-	expression tag
His 298	-	expression tag
His 299	-	expression tag
His 300	-	expression tag
His 301	-	expression tag
His 302	-	expression tag
His 303	-	expression tag
His 304	-	expression tag
His 305	-	expression tag

S, T: 1 - 363 (UniProt: Q9KWT9)

PDB	External Database	Details
Gln 160	Glu 160	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	207620.9
Branched	Number of molecules	1
	Total formula weight	898.6
All*	Total formula weight	208519.6

*Water molecules are not included.