4XTC

Crystal structure of bacterial alginate ABC transporter in complex with alginate pentasaccharide-bound periplasmic protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL38B1
Synchrotron site	SPring-8
Beamline	BL38B1
Temperature [K]	100
Detector technology	CCD
Collection date	2014-12-12
Detector	RAYONIX MX225HE
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	71.608, 132.963, 272.973
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.420 - 3.600
R-factor	0.241
Rwork	0.238
R-free	0.28850
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4xig
RMSD bond length	0.003
RMSD bond angle	0.781
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.660
High resolution limit [Å]	3.600	3.600
Rmerge		0.461
Number of reflections	30118
<I/σ(I)>	9.2
Completeness [%]	95.6	98
Redundancy	4.9	5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	PEG3000, N-(2-acetamido)iminodiacetic acid, sodium formate, 3-[(3-cholamidopropyl)dimethylammonio]-2-hydroxypropanesulfonate