4XR2

Escherichia Coli Replication Terminator Protein (Tus) H114A mutant Complexed With DNA- TerA lock.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	DNA replication terminus site-binding protein	polymer	316	36736.2	1	UniProt (P16525) Pfam (PF05472)	Escherichia coli (strain K12)	Ter-binding protein
2	B (B)	DNA (5'-D(*TP*AP*GP*TP*TP*AP*CP*AP*AP*CP*AP*TP*AP*C)-3')	polymer	16	4856.2	1		Escherichia coli
3	C (C)	DNA (5'-D(*TP*GP*AP*TP*AP*TP*GP*TP*TP*GP*TP*AP*AP*CP*TP*A)-3')	polymer	16	4927.2	1		Escherichia coli
4	D, E (A)	IODIDE ION	non-polymer		126.9	2	Chemie (IOD)
5	F (A)	(4S)-2-METHYL-2,4-PENTANEDIOL	non-polymer		118.2	1	Chemie (MPD)
6	G (A)	1,2-ETHANEDIOL	non-polymer		62.1	1	Chemie (EDO)
7	H, I, J (A, B, C)	water	water		18.0	69	Chemie (HOH)

Sequence modifications

A: 1 - 309 (UniProt: P16525)

PDB	External Database	Details
Met -6	-	initiating methionine
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
Ala 144	His 144	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	46519.6
Non-Polymers*	Number of molecules	4
	Total formula weight	434.0
All*	Total formula weight	46953.7

*Water molecules are not included.