4XR2
Escherichia Coli Replication Terminator Protein (Tus) H114A mutant Complexed With DNA- TerA lock.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-10-15 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.95370 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 64.505, 64.505, 250.941 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 62.740 - 2.350 |
| R-factor | 0.22211 |
| Rwork | 0.220 |
| R-free | 0.26567 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2i05 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.709 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 75.000 | 2.430 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.080 | 0.856 |
| Number of reflections | 23147 | |
| <I/σ(I)> | 26.7 | 2 |
| Completeness [%] | 99.6 | 100 |
| Redundancy | 6.6 | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 8-12% (v/v) PEG 3350, 100 mM NaI, 50 mM Bis-Tris |
