4XH9

CRYSTAL STRUCTURE OF HUMAN RHOA IN COMPLEX WITH DH/PH FRAGMENT OF THE GUANINE NUCLEOTIDE EXCHANGE FACTOR NET1

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (A, D)	Neuroepithelial cell-transforming gene 1 protein	polymer	361	42232.4	2	UniProt (Q7Z628) Pfam (PF00621) Pfam (PF00169)	Homo sapiens (Human)	Proto-oncogene p65 Net1,Rho guanine nucleotide exchange factor 8
2	B, D (B, E)	Transforming protein RhoA	polymer	180	20289.2	2	UniProt (P61586) Pfam (PF00071)	Homo sapiens (Human)	Rho cDNA clone 12,h12
3	E, F, G, H (A, B, D, E)	water	water		18.0	921	Chemie (HOH)

Sequence modifications

A, D: 149 - 501 (UniProt: Q7Z628)

PDB	External Database	Details
Met 147	-	initiating methionine
Gly 148	-	expression tag
His 502	-	expression tag
His 503	-	expression tag
His 504	-	expression tag
His 505	-	expression tag
His 506	-	expression tag
His 507	-	expression tag

B, E: 2 - 180 (UniProt: P61586)

PDB	External Database	Details
Gly 1	-	expression tag
Asn 25	Phe 25	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	125043.1
All*	Total formula weight	125043.1

*Water molecules are not included.