4XD1

X-ray structure of the N-formyltransferase QdtF from Providencia alcalifaciens, W305A mutant, in the presence of TDP-Qui3N and N5-THF

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	TDP-3-aminoquinovose-N-formyltransferase	polymer	417	48048.6	1	UniProt (F8RC03) Pfam (PF00551) Pfam (PF12796) In PDB	Providencia alcalifaciens
2	A	N-{[4-({[(6R)-2-amino-5-formyl-4-oxo-1,4,5,6,7,8-hexahydropteridin-6-yl]methyl}amino)phenyl]carbonyl}-L-glutamic acid	non-polymer		473.4	1	Chemie (FON)
3	A	[(3R,4S,5S,6R)-4-amino-3,5-dihydroxy-6-methyloxan-2-yl][hydroxy-[[(2R,3S,5R)-3-hydroxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy]phosphoryl] hydrogen phosphate	non-polymer		547.3	1	Chemie (T3Q)
4	A	SODIUM ION	non-polymer		23.0	4	Chemie (NA)
5	A	POTASSIUM ION	non-polymer		39.1	1	Chemie (K)
6	A	1,2-ETHANEDIOL	non-polymer		62.1	5	Chemie (EDO)
7	A	CHLORIDE ION	non-polymer		35.5	3	Chemie (CL)
8		water	water		18.0	605	Chemie (HOH)

Sequence modifications

A: 1 - 397 (UniProt: F8RC03)

PDB	External Database	Details
Met -19	-	expression tag
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Glu -7	-	expression tag
Asn -6	-	expression tag
Leu -5	-	expression tag
Tyr -4	-	expression tag
Phe -3	-	expression tag
Gln -2	-	expression tag
Gly -1	-	expression tag
Ala 0	-	expression tag
Ala 305	Trp 305	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	48048.6
Non-Polymers*	Number of molecules	15
	Total formula weight	1568.5
All*	Total formula weight	49617.2

*Water molecules are not included.