4XB6

Structure of the E. coli C-P lyase core complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, E (A, E)	Alpha-D-ribose 1-methylphosphonate 5-triphosphate synthase subunit PhnG	polymer	150	16560.6	2	UniProt (P16685) Pfam (PF06754)	Escherichia coli str. K-12 substr. MG1655	RPnTP synthase subunit PhnG
2	B, F (B, F)	Alpha-D-ribose 1-methylphosphonate 5-triphosphate synthase subunit PhnH	polymer	194	21226.5	2	UniProt (P16686) Pfam (PF05845)	Escherichia coli str. K-12 substr. MG1655	RPnTP synthase subunit PhnH
3	C, G (C, G)	Alpha-D-ribose 1-methylphosphonate 5-triphosphate synthase subunit PhnI	polymer	354	38922.7	2	UniProt (P16687) Pfam (PF05861)	Escherichia coli str. K-12 substr. MG1655	RPnTP synthase subunit PhnI,Ribose 1-methylphosphonate 5-triphosphate synthase nucleosidase subunit
4	D, H (D, H)	Alpha-D-ribose 1-methylphosphonate 5-phosphate C-P lyase	polymer	281	31879.1	2	UniProt (P16688) Pfam (PF06007)	Escherichia coli str. K-12 substr. MG1655	PRPn C-P lyase
5	I, K, M, O (C, D, G, H)	SULFATE ION	non-polymer		96.1	4	Chemie (SO4)
6	J, L, N, P (C, D, G, H)	ZINC ION	non-polymer		65.4	4	Chemie (ZN)
7	Q, R, S, T, U... (A, B, C, D, E...)	water	water		18.0	1792	Chemie (HOH)

Sequence modifications

B, F: 1 - 194 (UniProt: P16686)

PDB	External Database	Details
Arg 152	Gln 152	engineered mutation

C, G: 1 - 354 (UniProt: P16687)

PDB	External Database	Details
Val 322	Ala 322	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	217177.9
Non-Polymers*	Number of molecules	8
	Total formula weight	645.9
All*	Total formula weight	217823.8

*Water molecules are not included.