4XB2

Hyperthermophilic archaeal homoserine dehydrogenase mutant in complex with NADPH

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	319aa long hypothetical homoserine dehydrogenase	polymer	335	36916.9	2	UniProt (O58802) Pfam (PF03447) Pfam (PF00742)	Pyrococcus horikoshii OT3
2	C, F (A, B)	NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE	non-polymer		745.4	2	Chemie (NDP)
3	D, G (A, B)	L-HOMOSERINE	non-polymer		119.1	2	Chemie (HSE)
4	E, H (A, B)	SODIUM ION	non-polymer		23.0	2	Chemie (NA)
5	I, J (A, B)	water	water		18.0	211	Chemie (HOH)

Sequence modifications

A, B: 1 - 319 (UniProt: O58802)

PDB	External Database	Details
Met -15	-	expression tag
Asn -14	-	expression tag
His -13	-	expression tag
Lys -12	-	expression tag
Val -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
Ile -4	-	expression tag
Glu -3	-	expression tag
Gly -2	-	expression tag
Arg -1	-	expression tag
His 0	-	expression tag
Ala 57	Lys 57	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	73833.8
Non-Polymers*	Number of molecules	6
	Total formula weight	1775.1
All*	Total formula weight	75608.8

*Water molecules are not included.