4XB2
Hyperthermophilic archaeal homoserine dehydrogenase mutant in complex with NADPH
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2014-08-28 |
Detector | RIGAKU RAXIS VII |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 78.389, 47.811, 90.534 |
Unit cell angles | 90.00, 95.54, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.430 |
R-factor | 0.1672 |
Rwork | 0.164 |
R-free | 0.22340 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4xb1 |
RMSD bond length | 0.014 |
RMSD bond angle | 1.748 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | DM |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.520 |
High resolution limit [Å] | 2.430 | 5.230 | 2.430 |
Rmerge | 0.045 | 0.024 | 0.175 |
Total number of observations | 102119 | ||
Number of reflections | 25243 | ||
<I/σ(I)> | 15 | 4.2 | |
Completeness [%] | 99.0 | 98.4 | 95.7 |
Redundancy | 4 | 4 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | PEGME2000, citrate |