4X57

Structure of an Arabidopsis E2 / Membrane-anchored Ubiquitin-fold Protein Complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (A, C)	Ubiquitin-conjugating enzyme E2 8	polymer	179	19819.7	2	UniProt (P35131) Pfam (PF00179)	Arabidopsis thaliana (Mouse-ear cress)	UBCAT4A,Ubiquitin carrier protein 8,Ubiquitin-conjugating enzyme E2-17 kDa 8,Ubiquitin-protein ligase 8
2	B, D (B, D)	Membrane-anchored ubiquitin-fold protein 3	polymer	138	15026.4	2	UniProt (Q9SW27) Pfam (PF13881)	Arabidopsis thaliana (Mouse-ear cress)	Membrane-anchored ub-fold protein 3,ATGP4
3	E, F, G, H, I... (A, C, D)	SULFATE ION	non-polymer		96.1	6	Chemie (SO4)

Sequence modifications

A, C: 1 - 148 (UniProt: P35131)

PDB	External Database	Details
Met -30	-	initiating methionine
Gly -29	-	expression tag
Ser -28	-	expression tag
Ser -27	-	expression tag
His -26	-	expression tag
His -25	-	expression tag
His -24	-	expression tag
His -23	-	expression tag
His -22	-	expression tag
His -21	-	expression tag
Gly -20	-	expression tag
Thr -19	-	expression tag
Gly -18	-	expression tag
Ser -17	-	expression tag
Tyr -16	-	expression tag
Ile -15	-	expression tag
Thr -14	-	expression tag
Ser -13	-	expression tag
Leu -12	-	expression tag
Tyr -11	-	expression tag
Lys -10	-	expression tag
Lys -9	-	expression tag
Ala -8	-	expression tag
Gly -7	-	expression tag
Ser -6	-	expression tag
Ala -5	-	expression tag
Ala -4	-	expression tag
Ala -3	-	expression tag
Pro -2	-	expression tag
Phe -1	-	expression tag
Thr 0	-	expression tag

B, D: 1 - 118 (UniProt: Q9SW27)

PDB	External Database	Details
Met -19	-	initiating methionine
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	69692.2
Non-Polymers*	Number of molecules	6
	Total formula weight	576.4
All*	Total formula weight	70268.6

*Water molecules are not included.