4X4U

Crystal structure of the A.fulgidus CCA-adding enzyme in complex with a human MenBeta minihelix ending in CCACC

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C, E, F (A, C, E, F)	CCA-adding enzyme	polymer	457	53672.5	4	UniProt (O28126) Pfam (PF01909) Pfam (PF09249) Pfam (PF21133)	Archaeoglobus fulgidus	CCA tRNA nucleotidyltransferase,tRNA CCA-pyrophosphorylase,tRNA adenylyl-/cytidylyl- transferase,tRNA nucleotidyltransferase,tRNA-NT
2	B, D, G (B, D, G)	human MenBeta minihelix	polymer	37	11886.1	3		Homo sapiens
3	H (H)	RNA (5'-D(*GP*G)-3')	polymer	2	645.5	1		Homo sapiens
4	I, P (A, F)	DIPHOSPHOMETHYLPHOSPHONIC ACID ADENOSYL ESTER	non-polymer		505.2	2	Chemie (APC)
5	J, K, L, N, Q (A, E, H)	GLYCEROL	non-polymer		92.1	5	Chemie (GOL)
6	M, O (C, E)	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	2	Chemie (PEG)
7	R, S, T, U, V... (A, B, C, E, F...)	water	water		18.0	99	Chemie (HOH)

Sequence modifications

A, C, E, F: 1 - 437 (UniProt: O28126)

PDB	External Database	Details
Ser 438	-	expression tag
Asn 439	-	expression tag
Ser 440	-	expression tag
Ser 441	-	expression tag
Ser 442	-	expression tag
Val 443	-	expression tag
Asp 444	-	expression tag
Lys 445	-	expression tag
Leu 446	-	expression tag
Ala 447	-	expression tag
Ala 448	-	expression tag
Ala 449	-	expression tag
Leu 450	-	expression tag
Glu 451	-	expression tag
His 452	-	expression tag
His 453	-	expression tag
His 454	-	expression tag
His 455	-	expression tag
His 456	-	expression tag
His 457	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	250993.6
Non-Polymers*	Number of molecules	9
	Total formula weight	1683.1
All*	Total formula weight	252676.7

*Water molecules are not included.