4X4U

Crystal structure of the A.fulgidus CCA-adding enzyme in complex with a human MenBeta minihelix ending in CCACC

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000049	molecular_function	tRNA binding
A	0000287	molecular_function	magnesium ion binding
A	0001680	biological_process	tRNA 3'-terminal CCA addition
A	0003723	molecular_function	RNA binding
A	0004810	molecular_function	CCA tRNA nucleotidyltransferase activity
A	0005524	molecular_function	ATP binding
A	0008033	biological_process	tRNA processing
A	0016779	molecular_function	nucleotidyltransferase activity
A	0031123	biological_process	RNA 3'-end processing
A	0042245	biological_process	RNA repair
A	0046872	molecular_function	metal ion binding
A	0106354	biological_process	tRNA surveillance
A	0160016	molecular_function	CCACCA tRNA nucleotidyltransferase activity
C	0000049	molecular_function	tRNA binding
C	0000287	molecular_function	magnesium ion binding
C	0001680	biological_process	tRNA 3'-terminal CCA addition
C	0003723	molecular_function	RNA binding
C	0004810	molecular_function	CCA tRNA nucleotidyltransferase activity
C	0005524	molecular_function	ATP binding
C	0008033	biological_process	tRNA processing
C	0016779	molecular_function	nucleotidyltransferase activity
C	0031123	biological_process	RNA 3'-end processing
C	0042245	biological_process	RNA repair
C	0046872	molecular_function	metal ion binding
C	0106354	biological_process	tRNA surveillance
C	0160016	molecular_function	CCACCA tRNA nucleotidyltransferase activity
E	0000049	molecular_function	tRNA binding
E	0000287	molecular_function	magnesium ion binding
E	0001680	biological_process	tRNA 3'-terminal CCA addition
E	0003723	molecular_function	RNA binding
E	0004810	molecular_function	CCA tRNA nucleotidyltransferase activity
E	0005524	molecular_function	ATP binding
E	0008033	biological_process	tRNA processing
E	0016779	molecular_function	nucleotidyltransferase activity
E	0031123	biological_process	RNA 3'-end processing
E	0042245	biological_process	RNA repair
E	0046872	molecular_function	metal ion binding
E	0106354	biological_process	tRNA surveillance
E	0160016	molecular_function	CCACCA tRNA nucleotidyltransferase activity
F	0000049	molecular_function	tRNA binding
F	0000287	molecular_function	magnesium ion binding
F	0001680	biological_process	tRNA 3'-terminal CCA addition
F	0003723	molecular_function	RNA binding
F	0004810	molecular_function	CCA tRNA nucleotidyltransferase activity
F	0005524	molecular_function	ATP binding
F	0008033	biological_process	tRNA processing
F	0016779	molecular_function	nucleotidyltransferase activity
F	0031123	biological_process	RNA 3'-end processing
F	0042245	biological_process	RNA repair
F	0046872	molecular_function	metal ion binding
F	0106354	biological_process	tRNA surveillance
F	0160016	molecular_function	CCACCA tRNA nucleotidyltransferase activity

Functional Information from PDB Data

site_id	AC1
Number of Residues	14
Details	binding site for residue APC A 501

Chain	Residue
A	GLY46
A	TYR173
A	ARG224
B	C34
B	C36
B	C37
A	SER47
A	ARG50
A	GLU59
A	ASP61
A	THR130
A	HIS133
A	LYS152
A	TYR161

---

site_id	AC2
Number of Residues	3
Details	binding site for residue GOL A 502

Chain	Residue
A	GLU311
A	ARG380
A	HOH612

---

site_id	AC3
Number of Residues	2
Details	binding site for residue GOL A 503

Chain	Residue
A	VAL355
A	LYS356

---

site_id	AC4
Number of Residues	3
Details	binding site for residue PEG C 501

Chain	Residue
C	GLU311
C	ARG380
C	LYS381

---

site_id	AC5
Number of Residues	4
Details	binding site for residue GOL E 501

Chain	Residue
A	ARG361
B	U14
E	ARG284
E	TYR411

---

site_id	AC6
Number of Residues	7
Details	binding site for residue PEG E 502

Chain	Residue
A	ARG361
A	ASN362
A	ARG363
E	SER394
E	ARG409
E	TYR411
E	PHE412

---

site_id	AC7
Number of Residues	13
Details	binding site for residue APC F 501

Chain	Residue
F	GLY46
F	ARG50
F	THR130
F	HIS133
F	TYR161
F	GLY162
F	ALA163
F	GLU164
F	SER171
F	GLY172
F	TYR173
F	ARG224
G	G9

---

site_id	AC8
Number of Residues	5
Details	binding site for residue GOL H 101

Chain	Residue
A	ARG344
B	U13
B	U14
B	A17
H	G2

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Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	4
Details	BINDING: BINDING => ECO:0000269|PubMed:14592988, ECO:0000269|PubMed:14636575, ECO:0000269|PubMed:25640237, ECO:0007744|PDB:1R89, ECO:0007744|PDB:1UEU, ECO:0007744|PDB:4X4O, ECO:0007744|PDB:4X4Q, ECO:0007744|PDB:4X4S

Chain	Residue	Details
A	SER47
C	SER47
E	SER47
F	SER47

---

site_id	SWS_FT_FI2
Number of Residues	16
Details	BINDING: BINDING => ECO:0000269|PubMed:14592988, ECO:0000269|PubMed:14636575, ECO:0000269|PubMed:25640237, ECO:0007744|PDB:1R89, ECO:0007744|PDB:1UEU, ECO:0007744|PDB:4X4Q, ECO:0007744|PDB:4X4S

Chain	Residue	Details
A	ARG50
E	HIS133
E	LYS152
E	TYR161
F	ARG50
F	HIS133
F	LYS152
F	TYR161
A	HIS133
A	LYS152
A	TYR161
C	ARG50
C	HIS133
C	LYS152
C	TYR161
E	ARG50

---

site_id	SWS_FT_FI3
Number of Residues	4
Details	BINDING: BINDING => ECO:0000269|PubMed:14592988, ECO:0007744|PDB:1UEV

Chain	Residue	Details
A	GLU59
C	GLU59
E	GLU59
F	GLU59

---

site_id	SWS_FT_FI4
Number of Residues	8
Details	BINDING: BINDING => ECO:0000269|PubMed:14592988, ECO:0007744|PDB:1R89, ECO:0007744|PDB:1UEV

Chain	Residue	Details
A	ASP61
A	ASP110
C	ASP61
C	ASP110
E	ASP61
E	ASP110
F	ASP61
F	ASP110

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