4X4T

Crystal structure of the A.fulgidus CCA-adding enzyme in complex with a G70A arginyl-tRNA minihelix ending in CCACCA

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C, E, F	CCA-adding enzyme	polymer	457	53672.5	4	UniProt (O28126) Pfam (PF01909) Pfam (PF09249) Pfam (PF21133) In PDB	Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)	CCA tRNA nucleotidyltransferase,tRNA CCA-pyrophosphorylase,tRNA adenylyl-/cytidylyl- transferase,tRNA nucleotidyltransferase,tRNA-NT
2	B, D	G70A tRNA minihelix ending in CCACCA	polymer	34	11149.2	2		Homo sapiens
3	G	G70A tRNA minihelix ending in CCACCA	polymer	31	10193.6	1		Homo sapiens
4	H	RNA (5'-D(P*CP*G)-3')	polymer	2	605.4	1		Homo sapiens
5	I	RNA (5'-D(P*GP*G)-3')	polymer	2	645.5	1		Homo sapiens
6	A	GLYCEROL	non-polymer		92.1	3	Chemie (GOL)
7	E	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	1	Chemie (PEG)
8		water	water		18.0	357	Chemie (HOH)

Sequence modifications

A, C, E, F: 1 - 437 (UniProt: O28126)

PDB	External Database	Details
Ser 438	-	expression tag
Asn 439	-	expression tag
Ser 440	-	expression tag
Ser 441	-	expression tag
Ser 442	-	expression tag
Val 443	-	expression tag
Asp 444	-	expression tag
Lys 445	-	expression tag
Leu 446	-	expression tag
Ala 447	-	expression tag
Ala 448	-	expression tag
Ala 449	-	expression tag
Leu 450	-	expression tag
Glu 451	-	expression tag
His 452	-	expression tag
His 453	-	expression tag
His 454	-	expression tag
His 455	-	expression tag
His 456	-	expression tag
His 457	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	9
	Total formula weight	248432.9
Non-Polymers*	Number of molecules	4
	Total formula weight	382.4
All*	Total formula weight	248815.3

*Water molecules are not included.