4X4R

Crystal structure of the A.fulgidus CCA-adding enzyme in complex with a G70A arginyl-tRNA minihelix ending in CCACC and AMPcPP

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (A, C)	CCA-adding enzyme	polymer	457	53672.5	2	UniProt (O28126) Pfam (PF01909) Pfam (PF09249) Pfam (PF21133)	Archaeoglobus fulgidus	CCA tRNA nucleotidyltransferase,tRNA CCA-pyrophosphorylase,tRNA adenylyl-/cytidylyl- transferase,tRNA nucleotidyltransferase,tRNA-NT
2	B, D (B, D)	G70A tRNA minihelix ending in CCACC	polymer	37	11868.1	2		Homo sapiens
3	E (H)	RNA (5'-D(*CP*G)-3')	polymer	2	605.4	1		Homo sapiens
4	F, K (A, C)	DIPHOSPHOMETHYLPHOSPHONIC ACID ADENOSYL ESTER	non-polymer		505.2	2	Chemie (APC)
5	G, L (A, C)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
6	H (A)	2-(N-MORPHOLINO)-ETHANESULFONIC ACID	non-polymer		195.2	1	Chemie (MES)
7	I, J, M (A, C)	GLYCEROL	non-polymer		92.1	3	Chemie (GOL)
8	N (C)	D(-)-TARTARIC ACID	non-polymer		150.1	1	Chemie (TAR)
9	O (C)	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	1	Chemie (PEG)

Sequence modifications

A, C: 1 - 437 (UniProt: O28126)

PDB	External Database	Details
Ser 438	-	expression tag
Asn 439	-	expression tag
Ser 440	-	expression tag
Ser 441	-	expression tag
Ser 442	-	expression tag
Val 443	-	expression tag
Asp 444	-	expression tag
Lys 445	-	expression tag
Leu 446	-	expression tag
Ala 447	-	expression tag
Ala 448	-	expression tag
Ala 449	-	expression tag
Leu 450	-	expression tag
Glu 451	-	expression tag
His 452	-	expression tag
His 453	-	expression tag
His 454	-	expression tag
His 455	-	expression tag
His 456	-	expression tag
His 457	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	131686.6
Non-Polymers*	Number of molecules	10
	Total formula weight	1786.8
All*	Total formula weight	133473.3

*Water molecules are not included.