4X4R
Crystal structure of the A.fulgidus CCA-adding enzyme in complex with a G70A arginyl-tRNA minihelix ending in CCACC and AMPcPP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-11-09 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.1 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 111.100, 217.368, 58.562 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.906 - 3.202 |
R-factor | 0.2116 |
Rwork | 0.209 |
R-free | 0.26640 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.003 |
RMSD bond angle | 0.684 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 30.000 | 30.000 | 3.260 |
High resolution limit [Å] | 3.200 | 8.620 | 3.200 |
Rmerge | 0.161 | 0.097 | 0.380 |
Total number of observations | 84436 | ||
Number of reflections | 24077 | ||
<I/σ(I)> | 4.6 | ||
Completeness [%] | 99.7 | 99.6 | 99.8 |
Redundancy | 3.5 | 3.2 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.8 | 290 | 24% PEG3350, 0.2M NaK tartrate, 0.1M MES 6.8 |