4X4O

Crystal structure of the A.fulgidus CCA-adding enzyme in complex with a G70A arginyl-tRNA minihelix and CTP

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (A, C)	CCA-adding enzyme	polymer	457	53672.5	2	UniProt (O28126) Pfam (PF01909) Pfam (PF09249) Pfam (PF21133)	Archaeoglobus fulgidus	CCA tRNA nucleotidyltransferase,tRNA CCA-pyrophosphorylase,tRNA adenylyl-/cytidylyl- transferase,tRNA nucleotidyltransferase,tRNA-NT
2	B, D (B, D)	G70A tRNA minihelix	polymer	34	10912.5	2		Homo sapiens
3	E, I, K (A, C, D)	CYTIDINE-5'-TRIPHOSPHATE	non-polymer		483.2	3	Chemie (CTP)
4	F, G, H, J, L... (A, B, C, D)	MANGANESE (II) ION	non-polymer		54.9	8	Chemie (MN)
5	P (C)	water	water		18.0	2	Chemie (HOH)

Sequence modifications

A, C: 1 - 437 (UniProt: O28126)

PDB	External Database	Details
Ser 438	-	expression tag
Asn 439	-	expression tag
Ser 440	-	expression tag
Ser 441	-	expression tag
Ser 442	-	expression tag
Val 443	-	expression tag
Asp 444	-	expression tag
Lys 445	-	expression tag
Leu 446	-	expression tag
Ala 447	-	expression tag
Ala 448	-	expression tag
Ala 449	-	expression tag
Leu 450	-	expression tag
Glu 451	-	expression tag
His 452	-	expression tag
His 453	-	expression tag
His 454	-	expression tag
His 455	-	expression tag
His 456	-	expression tag
His 457	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	129170.0
Non-Polymers*	Number of molecules	11
	Total formula weight	1889.0
All*	Total formula weight	131059.0

*Water molecules are not included.