4X20
Discovery of cytotoxic Dolastatin 10 analogs with N-terminal modifications
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, C (A, C) | Tubulin alpha chain | polymer | 451 | 50188.4 | 2 | UniProt (D0VWZ0) Pfam (PF00091) Pfam (PF03953) | Ovis aries (Sheep) | |
| 2 | B, D (B, D) | Tubulin beta chain | polymer | 445 | 49969.8 | 2 | UniProt (D0VWY9) Pfam (PF00091) Pfam (PF03953) | Ovis aries (Sheep) | |
| 3 | E (E) | Stathmin-4 | polymer | 142 | 16719.9 | 1 | UniProt (P63043) Pfam (PF00836) | Rattus norvegicus (Rat) | Stathmin-like protein B3,RB3 |
| 4 | F, K (A, C) | GUANOSINE-5'-TRIPHOSPHATE | non-polymer | 523.2 | 2 | Chemie (GTP) | |||
| 5 | G, L (A, C) | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) | |||
| 6 | H, M (B, D) | GUANOSINE-5'-DIPHOSPHATE | non-polymer | 443.2 | 2 | Chemie (GDP) | |||
| 7 | I, N (B, D) | N-[(7S)-1,2,3,10-tetramethoxy-9-oxo-6,7-dihydro-5H-benzo[d]heptalen-7-yl]ethanamide | non-polymer | 399.4 | 2 | Chemie (LOC) | |||
| 8 | J, O (B, D) | 2-methyl-L-prolyl-N-[(3R,4S,5S)-1-{(2S)-2-[(1R,2R)-3-{[(1S)-1-carboxy-2-phenylethyl]amino}-1-methoxy-2-methyl-3-oxopropyl]pyrrolidin-1-yl}-3-methoxy-5-methyl-1-oxoheptan-4-yl]-N-methyl-L-valinamide | non-polymer | 729.9 | 2 | BIRD (PRD_002153) Chemie (3WY) |
Sequence modifications
E: 5 - 145 (UniProt: P63043)
| PDB | External Database | Details |
|---|---|---|
| Ala 4 | - | expression tag |
| Ala 14 | Cys 58 | engineered mutation |
| Trp 20 | Phe 64 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 5 |
| Total formula weight | 217036.4 | |
| Non-Polymers* | Number of molecules | 10 |
| Total formula weight | 4240.1 | |
| All* | Total formula weight | 221276.6 |
