4X0F
Crystal structure of crosslink stabilized long-form PDE4B in complex with (R)-(-)-rolipram
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | cAMP-specific 3',5'-cyclic phosphodiesterase 4B | polymer | 655 | 74791.4 | 2 | UniProt (Q07343) Pfam (PF18100) Pfam (PF00233) | Homo sapiens (Human) | DPDE4,PDE32 |
| 2 | C, D, H (A, B) | ZINC ION | non-polymer | 65.4 | 3 | Chemie (ZN) | |||
| 3 | E, I (A, B) | IODIDE ION | non-polymer | 126.9 | 2 | Chemie (IOD) | |||
| 4 | F, J (A, B) | ROLIPRAM | non-polymer | 275.3 | 2 | Chemie (ROL) | |||
| 5 | G, K (A, B) | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) |
Sequence modifications
A, B: 122 - 736 (UniProt: Q07343)
| PDB | External Database | Details |
|---|---|---|
| Met 82 | - | initiating methionine |
| Ala 83 | - | expression tag |
| Gly 84 | - | expression tag |
| Leu 85 | - | expression tag |
| Asn 86 | - | expression tag |
| Asp 87 | - | expression tag |
| Ile 88 | - | expression tag |
| Phe 89 | - | expression tag |
| Glu 90 | - | expression tag |
| Ala 91 | - | expression tag |
| Gln 92 | - | expression tag |
| Lys 93 | - | expression tag |
| Ile 94 | - | expression tag |
| Glu 95 | - | expression tag |
| Trp 96 | - | expression tag |
| His 97 | - | expression tag |
| Glu 98 | - | expression tag |
| Asn 99 | - | expression tag |
| Leu 100 | - | expression tag |
| Tyr 101 | - | expression tag |
| Phe 102 | - | expression tag |
| Gln 103 | - | expression tag |
| Gly 104 | - | expression tag |
| Ser 105 | - | expression tag |
| Asp 106 | - | expression tag |
| Tyr 107 | - | expression tag |
| Lys 108 | - | expression tag |
| Asp 109 | - | expression tag |
| Asp 110 | - | expression tag |
| Asp 111 | - | expression tag |
| Asp 112 | - | expression tag |
| Lys 113 | - | expression tag |
| Asp 114 | - | expression tag |
| Leu 115 | - | expression tag |
| Val 116 | - | expression tag |
| Pro 117 | - | expression tag |
| Arg 118 | - | expression tag |
| Gly 119 | - | expression tag |
| Ser 120 | - | expression tag |
| Met 121 | - | expression tag |
| Ala 133 | Ser 133 | engineered mutation |
| Ala 229 | Cys 229 | engineered mutation |
| Cys 267 | Ser 267 | engineered mutation |
| Ala 366 | Cys 366 | engineered mutation |
| Ala 492 | Cys 492 | engineered mutation |
| Ala 562 | Cys 562 | engineered mutation |
| Ala 604 | Cys 604 | engineered mutation |
| Cys 610 | Ser 610 | engineered mutation |
| Ala 654 | Ser 654 | engineered mutation |
| Ala 659 | Ser 659 | engineered mutation |
| Ala 661 | Ser 661 | engineered mutation |
| Ala 671 | Cys 671 | engineered mutation |
| Ala 709 | Cys 709 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 149582.8 | |
| Non-Polymers* | Number of molecules | 9 |
| Total formula weight | 1049.3 | |
| All* | Total formula weight | 150632.1 |
