4WZM

Mutant K18E of RNA dependent RNA polymerase from Foot-and-Mouth Disease Virus complexed with RNA

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	RNA dependent RNA polymerase	polymer	481	54110.3	1	UniProt (P03311) Pfam (PF00680)	Foot-and-mouth disease virus (FMDV)
2	B (B)	RNA template	polymer	8	2541.6	1		synthetic construct
3	C (C)	RNA primer	polymer	7	2212.4	1		synthetic construct
4	D, E (A)	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
5	F (A)	ACETATE ION	non-polymer		59.0	1	Chemie (ACT)
6	G (A)	MANGANESE (II) ION	non-polymer		54.9	1	Chemie (MN)
7	H, I, J (A, B, C)	water	water		18.0	35	Chemie (HOH)

Sequence modifications

A: 1 - 470 (UniProt: P03311)

PDB	External Database	Details
Glu 18	Lys 1875	engineered mutation
Ala 471	-	expression tag
Ala 472	-	expression tag
Ala 473	-	expression tag
Leu 474	-	expression tag
Glu 475	-	expression tag
His 476	-	expression tag
His 477	-	expression tag
His 478	-	expression tag
His 479	-	expression tag
His 480	-	expression tag
His 481	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	58864.2
Non-Polymers*	Number of molecules	4
	Total formula weight	298.2
All*	Total formula weight	59162.4

*Water molecules are not included.