4WZM
Mutant K18E of RNA dependent RNA polymerase from Foot-and-Mouth Disease Virus complexed with RNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-07-21 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.7 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 94.306, 94.306, 100.815 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 81.670 - 2.520 |
R-factor | 0.22623 |
Rwork | 0.224 |
R-free | 0.26156 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1wne |
RMSD bond length | 0.005 |
RMSD bond angle | 0.946 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | REFMAC |
Refinement software | REFMAC (5.8.0069) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 63.630 | 2.710 |
High resolution limit [Å] | 2.570 | 2.570 |
Rmerge | 0.065 | 0.327 |
Number of reflections | 18038 | |
<I/σ(I)> | 9.3 | 2.8 |
Completeness [%] | 99.0 | 97.5 |
Redundancy | 2.8 | 2.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 30% PEG 4K; 0.2M Mg(CH3COO)2, 0.1M MES pH6.0; 4% gamma- butyrolactone |