4WZM
Mutant K18E of RNA dependent RNA polymerase from Foot-and-Mouth Disease Virus complexed with RNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-07-21 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.7 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 94.306, 94.306, 100.815 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 81.670 - 2.520 |
| R-factor | 0.22623 |
| Rwork | 0.224 |
| R-free | 0.26156 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1wne |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.946 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0069) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 63.630 | 2.710 |
| High resolution limit [Å] | 2.570 | 2.570 |
| Rmerge | 0.065 | 0.327 |
| Number of reflections | 18038 | |
| <I/σ(I)> | 9.3 | 2.8 |
| Completeness [%] | 99.0 | 97.5 |
| Redundancy | 2.8 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 30% PEG 4K; 0.2M Mg(CH3COO)2, 0.1M MES pH6.0; 4% gamma- butyrolactone |
