4WXY

PLPS (inactive glutaminase mutant) co-crystallized with glutamine and R5P.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C, E, G, I...	Pyridoxal biosynthesis lyase PdxS	polymer	304	33044.9	6	UniProt (Q5L3Y2) Pfam (PF01680) In PDB	Geobacillus kaustophilus
2	B, D, F, H, J...	Glutamine amidotransferase subunit PdxT	polymer	228	24967.7	6	UniProt (Q5L3Y1) Pfam (PF01174) Pfam (PF00117) In PDB	Geobacillus kaustophilus	Glutamine amidotransferase glutaminase subunit PdxT
3		water	water		18.0	263	Chemie (HOH)

Sequence modifications

A, C, E, G, I, K: 1 - 294 (UniProt: Q5L3Y2)

PDB	External Database	Details
Glu -9	-	expression tag
Asn -8	-	expression tag
Leu -7	-	expression tag
Thr -6	-	expression tag
Pro -5	-	expression tag
Gln -4	-	expression tag
His -3	-	expression tag
Met -2	-	expression tag
Ala -1	-	expression tag
Ser 0	-	expression tag
Thr 216	Ala 216	conflict

B, D, F, H, J, L: 1 - 196 (UniProt: Q5L3Y1)

PDB	External Database	Details
Met -31	-	initiating methionine
Gly -30	-	expression tag
Ser -29	-	expression tag
Ser -28	-	expression tag
His -27	-	expression tag
His -26	-	expression tag
His -25	-	expression tag
His -24	-	expression tag
His -23	-	expression tag
His -22	-	expression tag
Ser -21	-	expression tag
Ser -20	-	expression tag
Gly -19	-	expression tag
Leu -18	-	expression tag
Val -17	-	expression tag
Pro -16	-	expression tag
Arg -15	-	expression tag
Gly -14	-	expression tag
Ser -13	-	expression tag
Gly -12	-	expression tag
Thr -11	-	expression tag
Glu -10	-	expression tag
Asn -9	-	expression tag
Leu -8	-	expression tag
Tyr -7	-	expression tag
Phe -6	-	expression tag
Gln -5	-	expression tag
Gly -4	-	expression tag
His -3	-	expression tag
Met -2	-	expression tag
Ala -1	-	expression tag
Ser 0	-	expression tag
Ser 32	Pro 32	conflict
Asn 169	His 169	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	12
	Total formula weight	348075.4
All*	Total formula weight	348075.4

*Water molecules are not included.