4WXH
Carminomycin-4-O-methyltransferase (DnrK) variant (298Ser insert) in complex with S-adenosyl-L-homocysteine (SAH) and aclacinomycin T
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, B | Carminomycin 4-O-methyltransferase DnrK | polymer | 367 | 40198.5 | 2 | UniProt (Q06528) Pfam (PF00891) In PDB | Streptomyces peucetius | COMT,Anthracycline 4-O-methyltransferase |
2 | A, B | S-ADENOSYL-L-HOMOCYSTEINE | non-polymer | 384.4 | 2 | Chemie (SAH) | |||
3 | A, B | methyl (1R,2R,4S)-2-ethyl-2,5,7-trihydroxy-6,11-dioxo-4-{[2,3,6-trideoxy-3-(dimethylamino)-alpha-L-lyxo-hexopyranosyl]oxy}-1,2,3,4,6,11-hexahydrotetracene-1-carboxylate | non-polymer | 569.6 | 2 | Chemie (3VL) | |||
4 | A | (2R,3S)-1,4-DIMERCAPTOBUTANE-2,3-DIOL | non-polymer | 154.3 | 1 | Chemie (DTU) | |||
5 | A, B | SULFATE ION | non-polymer | 96.1 | 5 | Chemie (SO4) | |||
6 | water | water | 18.0 | 343 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 356 (UniProt: Q06528)
PDB | External Database | Details |
---|---|---|
Met -10 | - | initiating methionine |
Ala -9 | - | expression tag |
His -8 | - | expression tag |
His -7 | - | expression tag |
His -6 | - | expression tag |
His -5 | - | expression tag |
His -4 | - | expression tag |
His -3 | - | expression tag |
His -2 | - | expression tag |
Arg -1 | - | expression tag |
Ser 0 | - | expression tag |
Ser 297 | - | insertion |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 2 |
Total formula weight | 80397.0 | |
Non-Polymers* | Number of molecules | 10 |
Total formula weight | 2542.6 | |
All* | Total formula weight | 82939.6 |