4WU3

Structure of the PTP-like myo-inositol phosphatase from Mitsuokella multacida in complex with myo-inositol-(1,3,4,5)-tetrakisphosphate

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	MYO-INOSITOL PHOSPHOHYDROLASE	polymer	632	72448.0	4	UniProt (A3QMF6) Pfam (PF14566)	Mitsuokella multacida
2	E, F, K, Q, R... (A, B, C, D)	INOSITOL-(1,3,4,5)-TETRAKISPHOSPHATE	non-polymer		500.1	6	Chemie (4IP)
3	G, L, M, S, X... (A, B, C, D)	PHOSPHATE ION	non-polymer		95.0	6	Chemie (PO4)
4	AA, BA, H, I, J... (D, A, B, C)	GLYCEROL	non-polymer		92.1	12	Chemie (GOL)
5	CA, DA, EA, FA (A, B, C, D)	water	water		18.0	2262	Chemie (HOH)

Sequence modifications

A, B, C, D: 32 - 640 (UniProt: A3QMF6)

PDB	External Database	Details
Met 9	-	initiating methionine
Gly 10	-	expression tag
Ser 11	-	expression tag
Ser 12	-	expression tag
His 13	-	expression tag
His 14	-	expression tag
His 15	-	expression tag
His 16	-	expression tag
His 17	-	expression tag
His 18	-	expression tag
Ser 19	-	expression tag
Ser 20	-	expression tag
Gly 21	-	expression tag
Leu 22	-	expression tag
Val 23	-	expression tag
Pro 24	-	expression tag
Arg 25	-	expression tag
Gly 26	-	expression tag
Ser 27	-	expression tag
His 28	-	expression tag
Met 29	-	expression tag
Ala 30	-	expression tag
Ser 31	-	expression tag
Ser 250	Cys 250	engineered mutation
Ser 548	Cys 548	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	289792.1
Non-Polymers*	Number of molecules	24
	Total formula weight	4675.4
All*	Total formula weight	294467.5

*Water molecules are not included.