4WT9
APO CRYSTAL STRUCTURE OF HCV NS5B GENOTYPE 2A JFH-1 ISOLATE WITH E86Q E87Q S15G C223H V321I AND DELTA8 MUTATIONS
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | RNA-directed RNA polymerase | polymer | 572 | 63755.2 | 1 | UniProt (Q99IB8) Pfam (PF00998) | Hepatitis C virus JFH-1 | |
| 2 | B (A) | CHLORIDE ION | non-polymer | 35.5 | 1 | Chemie (CL) | |||
| 3 | C (A) | 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID | non-polymer | 238.3 | 1 | Chemie (EPE) | |||
| 4 | D (A) | 1-(2-METHOXY-ETHOXY)-2-{2-[2-(2-METHOXY-ETHOXY]-ETHOXY}-ETHANE | non-polymer | 266.3 | 1 | Chemie (PG6) | |||
| 5 | E (A) | water | water | 18.0 | 203 | Chemie (HOH) |
Sequence modifications
A: 1 - 570 (UniProt: Q99IB8)
| PDB | External Database | Details |
|---|---|---|
| Met -1 | - | expression tag |
| Ser 0 | - | expression tag |
| Gly 15 | Ser 2457 | engineered mutation |
| Gln 86 | Glu 2528 | engineered mutation |
| Gln 87 | Glu 2529 | engineered mutation |
| His 223 | Cys 2665 | engineered mutation |
| Ile 321 | Val 2763 | engineered mutation |
| - | Asn 2886 | deletion |
| - | Phe 2887 | deletion |
| - | Glu 2888 | deletion |
| - | Met 2889 | deletion |
| - | Tyr 2890 | deletion |
| - | Gly 2891 | deletion |
| - | Ser 2892 | deletion |
| - | Val 2893 | deletion |
| Gly 444 | Tyr 2894 | linker |
| Gly 445 | Ser 2895 | linker |
| Leu 571 | - | expression tag |
| Glu 572 | - | expression tag |
| His 573 | - | expression tag |
| His 574 | - | expression tag |
| His 575 | - | expression tag |
| His 576 | - | expression tag |
| His 577 | - | expression tag |
| His 578 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 63755.2 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 540.1 | |
| All* | Total formula weight | 64295.3 |
