4WSD

Complex of 70S ribosome with tRNA-Phe and mRNA with C-A mismatch in the second position in the A-site and with antibiotic paromomycin.

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, DB (13, 1G)	16S ribosomal RNA	polymer	1522	493997.3	2	GenBank (155076)	Thermus thermophilus
2	B, EB (1E, 12)	30S ribosomal protein S2	polymer	256	29317.7	2	UniProt (P80371) Pfam (PF00318)	Thermus thermophilus
3	C, FB (2E, 22)	30S ribosomal protein S3	polymer	239	26751.1	2	UniProt (P80372) Pfam (PF07650) Pfam (PF00189)	Thermus thermophilus
4	D, GB (3E, 32)	30S ribosomal protein S4	polymer	209	24373.4	2	UniProt (P80373) Pfam (PF00163) Pfam (PF01479)	Thermus thermophilus
5	E, HB (4E, 42)	30S ribosomal protein S5	polymer	162	17583.4	2	UniProt (Q5SHQ5) Pfam (PF00333) Pfam (PF03719)	Thermus thermophilus
6	F, IB (5E, 52)	30S ribosomal protein S6	polymer	101	11988.8	2	UniProt (Q5SLP8) Pfam (PF01250)	Thermus thermophilus	TS9
7	G, JB (6E, 62)	30S ribosomal protein S7	polymer	156	18051.0	2	UniProt (P17291) Pfam (PF00177)	Thermus thermophilus
8	H, KB (7E, 72)	30S ribosomal protein S8	polymer	138	15868.6	2	UniProt (Q5SHQ2) Pfam (PF00410) UniProt (by SIFTS) (P0DOY9)	Thermus thermophilus
9	I, LB (8E, 82)	30S ribosomal protein S9	polymer	128	14410.6	2	UniProt (P80374) Pfam (PF00380)	Thermus thermophilus
10	J, MB (1I, 1A)	30S ribosomal protein S10	polymer	105	11955.0	2	UniProt (Q5SHN7) Pfam (PF00338)	Thermus thermophilus
11	K, NB (2I, 2A)	30S ribosomal protein S11	polymer	129	13737.9	2	UniProt (P80376) Pfam (PF00411)	Thermus thermophilus
12	L, OB (3I, 3A)	30S ribosomal protein S12	polymer	132	14637.4	2	UniProt (Q5SHN3) Pfam (PF00164)	Thermus thermophilus
13	M, PB (4I, 4A)	30S ribosomal protein S13	polymer	126	14352.9	2	UniProt (P80377) Pfam (PF00416)	Thermus thermophilus
14	N, QB (5I, 5A)	30S ribosomal protein S14 type Z	polymer	61	7158.7	2	UniProt (Q5SHQ1) Pfam (PF00253) UniProt (by SIFTS) (P0DOY6)	Thermus thermophilus
15	O, RB (6I, 6A)	30S ribosomal protein S15	polymer	89	10578.4	2	UniProt (Q5SJ76) Pfam (PF00312)	Thermus thermophilus
16	P, SB (7I, 7A)	30S ribosomal protein S16	polymer	88	10410.0	2	UniProt (Q5SJH3) Pfam (PF00886)	Thermus thermophilus
17	Q, TB (8I, 8A)	30S ribosomal protein S17	polymer	105	12325.7	2	UniProt (Q5SHP7) Pfam (PF00366) UniProt (by SIFTS) (P0DOY7)	Thermus thermophilus
18	R, UB (9I, 9A)	30S ribosomal protein S18	polymer	88	10258.3	2	UniProt (Q5SLQ0) Pfam (PF01084)	Thermus thermophilus
19	S, VB (AI, AA)	30S ribosomal protein S19	polymer	93	10605.5	2	UniProt (Q5SHP2) Pfam (PF00203)	Thermus thermophilus
20	T, WB (BI, BA)	30S ribosomal protein S20	polymer	106	11736.1	2	UniProt (P80380) Pfam (PF01649)	Thermus thermophilus
21	U, XB (1F, 1B)	30S ribosomal protein Thx	polymer	27	3350.0	2	UniProt (Q5SIH3) Pfam (PF17070)	Thermus thermophilus	S31
22	V (1K)	tRNA-Phe	polymer	76	24629.8	1		Escherichia coli K-12
23	W, ZB (2K, 2L)	tRNA-fMet	polymer	77	24862.0	2		Escherichia coli K-12
24	X (3K)	tRNA-Phe	polymer	76	24599.7	1	GenBank (675820269)	Escherichia coli K-12
25	BC, Y (4K, 4L)	mRNA	polymer	30	9802.0	2		Thermus thermophilus HB8
26	CC, Z (1H, 14)	23S ribosomal RNA	polymer	2917	948539.9	2	GenBank (48268)	Thermus thermophilus
27	AA, DC (16, 1J)	5S ribosomal RNA	polymer	122	39540.6	2	GenBank (55771382)	Thermus thermophilus
28	BA, EC (11, 19)	50S ribosomal protein L2	polymer	276	30532.6	2	UniProt (P60405) Pfam (PF00181) Pfam (PF03947)	Thermus thermophilus
29	CA, FC (21, 29)	50S ribosomal protein L3	polymer	206	22450.2	2	UniProt (Q5SHN8) Pfam (PF00297)	Thermus thermophilus
30	DA, GC (31, 39)	50S ribosomal protein L4	polymer	210	23271.9	2	UniProt (Q5SHN9) Pfam (PF00573)	Thermus thermophilus	L1e
31	EA, HC (41, 49)	50S ribosomal protein L5	polymer	182	21061.6	2	UniProt (Q5SHQ0) Pfam (PF00281) Pfam (PF00673)	Thermus thermophilus
32	FA, IC (51, 59)	50S ribosomal protein L6	polymer	180	19568.9	2	UniProt (Q5SHQ3) Pfam (PF00347) UniProt (by SIFTS) (P0DOY8)	Thermus thermophilus
33	GA, JC (61, 69)	50S ribosomal protein L9	polymer	148	16422.2	2	UniProt (Q5SLQ1) Pfam (PF01281) Pfam (PF03948)	Thermus thermophilus
34	HA, KC (58, 15)	50S ribosomal protein L13	polymer	140	15927.9	2	UniProt (P60488) Pfam (PF00572)	Thermus thermophilus
35	IA, LC (68, 25)	50S ribosomal protein L14	polymer	122	13323.6	2	UniProt (Q5SHP8) Pfam (PF00238)	Thermus thermophilus
36	JA, MC (78, 35)	50S ribosomal protein L15	polymer	150	16319.1	2	UniProt (Q5SHQ7) Pfam (PF00828)	Thermus thermophilus
37	KA, NC (88, 45)	50S ribosomal protein L16	polymer	141	15993.9	2	UniProt (P60489) Pfam (PF00252)	Thermus thermophilus
38	LA, OC (98, 55)	50S ribosomal protein L17	polymer	118	13750.1	2	UniProt (Q9Z9H5) Pfam (PF01196)	Thermus thermophilus
39	MA, PC (A8, 65)	50S ribosomal protein L18	polymer	112	12639.8	2	UniProt (Q5SHQ4) Pfam (PF00861)	Thermus thermophilus	TL24
40	NA, QC (B8, 75)	50S ribosomal protein L19	polymer	146	17188.8	2	UniProt (P60490) Pfam (PF01245)	Thermus thermophilus
41	OA, RC (C8, 85)	50S ribosomal protein L20	polymer	118	13779.3	2	UniProt (P60491) Pfam (PF00453)	Thermus thermophilus
42	PA, SC (D8, 95)	50S ribosomal protein L21	polymer	101	11069.2	2	UniProt (P60492) Pfam (PF00829)	Thermus thermophilus
43	QA, TC (E8, A5)	50S ribosomal protein L22	polymer	113	12808.1	2	UniProt (Q5SHP3) Pfam (PF00237)	Thermus thermophilus
44	RA, UC (F8, B5)	50S ribosomal protein L23	polymer	96	10759.8	2	UniProt (Q5SHP0) Pfam (PF00276)	Thermus thermophilus
45	SA, VC (G8, C5)	50S ribosomal protein L24	polymer	110	12085.6	2	UniProt (Q5SHP9) Pfam (PF00467) Pfam (PF17136)	Thermus thermophilus
46	TA, WC (H8, D5)	50S ribosomal protein L25	polymer	206	23238.6	2	UniProt (Q5SHZ1) Pfam (PF01386) Pfam (PF14693)	Thermus thermophilus	TL5
47	UA, XC (I8, E5)	50S ribosomal protein L27	polymer	85	9557.1	2	UniProt (P60493) Pfam (PF01016)	Thermus thermophilus
48	VA, YC (J8, F5)	50S ribosomal protein L28	polymer	98	11004.2	2	UniProt (P60494) Pfam (PF00830)	Thermus thermophilus
49	WA, ZC (K8, G5)	50S ribosomal protein L29	polymer	72	8670.3	2	UniProt (Q5SHP6) Pfam (PF00831)	Thermus thermophilus
50	AD, XA (L8, H5)	50S ribosomal protein L30	polymer	60	6799.1	2	UniProt (Q5SHQ6) Pfam (PF00327)	Thermus thermophilus
51	BD, YA (M8, I5)	50S ribosomal protein L31	polymer	71	8300.5	2	UniProt (Q5SJE1) Pfam (PF01197)	Thermus thermophilus
52	CD, ZA (N8, J5)	50S ribosomal protein L32	polymer	60	6722.1	2	UniProt (P80339) Pfam (PF01783)	Thermus thermophilus
53	AB, DD (O8, K5)	50S ribosomal protein L33	polymer	54	6632.9	2	UniProt (P35871) Pfam (PF00471)	Thermus thermophilus
54	BB, ED (P8, L5)	50S ribosomal protein L34	polymer	49	6132.4	2	UniProt (P80340) Pfam (PF00468)	Thermus thermophilus
55	CB, FD (Q8, M5)	50S ribosomal protein L35	polymer	65	7506.2	2	UniProt (Q5SKU1) Pfam (PF01632)	Thermus thermophilus
56	YB (1L)	tRNA-Phe	polymer	76	24613.8	1	GenBank (675820269)	Escherichia coli K-12
57	AC (3L)	tRNA-Phe	polymer	76	24531.7	1		Escherichia coli K-12
58	AAA, ABA, ACA, ADA, AE... (1H, 13, 16, 1G, 3E...)	MAGNESIUM ION	non-polymer		24.3	1238	Chemie (MG)
59	NIA, YI (1G, 13)	PAROMOMYCIN	non-polymer		615.6	2	Chemie (PAR)
60	BJ, CZA, GJ, OIA, REA... (3E, C5, 5I, 32, G8...)	ZINC ION	non-polymer		65.4	6	Chemie (ZN)
61	AAB, BAB, CAB, DAB, EAB... (G8, I8, L8, P8, Q8...)	water	water		18.0	2004	Chemie (HOH)

Sequence modifications

4I, 4A: 1 - 126 (UniProt: P80377)

PDB	External Database	Details
Ala 119	Gly 119	conflict

I8, E5: 1 - 85 (UniProt: P60493)

PDB	External Database	Details
Ala 6	Gly 6	conflict
Ala 8	Gly 8	conflict

Contents of the asymmetric unit

Polymers	Number of chains	110
	Total formula weight	4497791.4
Non-Polymers*	Number of molecules	1246
	Total formula weight	31713.3
All*	Total formula weight	4529504.7

*Water molecules are not included.