4WSD

Complex of 70S ribosome with tRNA-Phe and mRNA with C-A mismatch in the second position in the A-site and with antibiotic paromomycin.

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-06-29
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.000
Spacegroup name	P 21 21 21
Unit cell lengths	209.400, 449.200, 621.200
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	255.475 - 2.950
Rwork	0.193
R-free	0.23490
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3TVF 3TVE
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	300.000		3.050
High resolution limit [Å]	2.950	100.000	2.950
Rmerge	0.300	0.031	4.193
Rmeas	0.302	0.034	4.266
Total number of observations	90425707
Number of reflections	1213341	27	114461
<I/σ(I)>	18.67	8.99	1.13
Completeness [%]	99.9	58.7	99.8
Redundancy	74.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	298	Tris-acetate, KSCN, PEG 550MME, PEG 20K