4WPV

Crystal Structure of E83A mutant of Mtb PEPCK in complex with Zn2+ and phosphate ion

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Phosphoenolpyruvate carboxykinase [GTP]	polymer	622	68988.7	1	UniProt (A5TYT6) Pfam (PF17297) Pfam (PF00821)	Mycobacterium tuberculosis	PEPCK
2	B (A)	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
3	C, D (A)	ZINC ION	non-polymer		65.4	2	Chemie (ZN)
4	E, F, G, H, I... (A)	PHOSPHATE ION	non-polymer		95.0	6	Chemie (PO4)
5	K (A)	water	water		18.0	826	Chemie (HOH)

Sequence modifications

A: 1 - 606 (UniProt: A5TYT6)

PDB	External Database	Details
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Ala -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Ala 83	Glu 83	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	68988.7
Non-Polymers*	Number of molecules	9
	Total formula weight	792.7
All*	Total formula weight	69781.4

*Water molecules are not included.