4WPV

Crystal Structure of E83A mutant of Mtb PEPCK in complex with Zn2+ and phosphate ion

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000166	molecular_function	nucleotide binding
A	0004611	molecular_function	phosphoenolpyruvate carboxykinase activity
A	0004613	molecular_function	phosphoenolpyruvate carboxykinase (GTP) activity
A	0005525	molecular_function	GTP binding
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0006094	biological_process	gluconeogenesis
A	0006107	biological_process	oxaloacetate metabolic process
A	0016829	molecular_function	lyase activity
A	0016831	molecular_function	carboxy-lyase activity
A	0017076	molecular_function	purine nucleotide binding
A	0019543	biological_process	propionate catabolic process
A	0030145	molecular_function	manganese ion binding
A	0033993	biological_process	response to lipid
A	0042594	biological_process	response to starvation
A	0046327	biological_process	glycerol biosynthetic process from pyruvate
A	0046872	molecular_function	metal ion binding
A	0071333	biological_process	cellular response to glucose stimulus

Functional Information from PDB Data

site_id	AC1
Number of Residues	10
Details	binding site for residue GOL A 701

Chain	Residue
A	ALA83
A	HOH1113
A	THR86
A	THR99
A	ASN100
A	ASN101
A	ARG234
A	LEU467
A	PRO468
A	HOH1016

---

site_id	AC2
Number of Residues	5
Details	binding site for residue ZN A 702

Chain	Residue
A	LYS229
A	HIS249
A	ASP296
A	PO4704
A	PO4705

---

site_id	AC3
Number of Residues	4
Details	binding site for residue ZN A 703

Chain	Residue
A	PO4704
A	PO4705
A	PO4706
A	PO4708

---

site_id	AC4
Number of Residues	12
Details	binding site for residue PO4 A 704

Chain	Residue
A	LYS229
A	HIS249
A	ASP296
A	ARG389
A	ZN702
A	ZN703
A	PO4705
A	PO4706
A	PO4708
A	PO4709
A	HOH1507
A	HOH1538

---

site_id	AC5
Number of Residues	13
Details	binding site for residue PO4 A 705

Chain	Residue
A	HIS249
A	PHE269
A	PRO270
A	SER271
A	LYS275
A	ASP296
A	ZN702
A	ZN703
A	PO4704
A	PO4706
A	PO4707
A	PO4708
A	HOH1508

---

site_id	AC6
Number of Residues	10
Details	binding site for residue PO4 A 706

Chain	Residue
A	SER271
A	ALA272
A	ARG377
A	ZN703
A	PO4704
A	PO4705
A	PO4708
A	HOH847
A	HOH1496
A	HOH1500

---

site_id	AC7
Number of Residues	12
Details	binding site for residue PO4 A 707

Chain	Residue
A	ARG81
A	HIS249
A	PRO270
A	SER271
A	ARG460
A	ASP462
A	ALA465
A	PRO468
A	PHE469
A	PO4705
A	HOH1508
A	HOH1543

---

site_id	AC8
Number of Residues	11
Details	binding site for residue PO4 A 708

Chain	Residue
A	ARG81
A	SER271
A	ARG377
A	ARG389
A	ZN703
A	PO4704
A	PO4705
A	PO4706
A	PO4709
A	HOH1503
A	HOH1508

---

site_id	AC9
Number of Residues	7
Details	binding site for residue PO4 A 709

Chain	Residue
A	ARG81
A	GLY222
A	LYS229
A	ARG389
A	PO4704
A	PO4708
A	HOH1501

---

Functional Information from PROSITE/UniProt

site_id	PS00505
Number of Residues	9
Details	PEPCK_GTP Phosphoenolpyruvate carboxykinase (GTP) signature. FPSACGKTN

Chain	Residue	Details
A	PHE269-ASN277

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	1
Details	ACT_SITE: ACT_SITE => ECO:0000250|UniProtKB:P07379, ECO:0000255|HAMAP-Rule:MF_00452

Chain	Residue	Details
A	CYS273

---

site_id	SWS_FT_FI2
Number of Residues	5
Details	BINDING: BINDING => ECO:0000255|HAMAP-Rule:MF_00452, ECO:0000269|Ref.2

Chain	Residue	Details
A	ARG81
A	LYS229
A	HIS249
A	ASP296
A	ASN387

---

site_id	SWS_FT_FI3
Number of Residues	6
Details	BINDING: BINDING => ECO:0000250|UniProtKB:P07379, ECO:0000255|HAMAP-Rule:MF_00452

Chain	Residue	Details
A	TYR220
A	SER271
A	ALA272
A	ARG389
A	ARG420
A	PHE515

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