4WJ3

Crystal structure of the asparagine transamidosome from Pseudomonas aeruginosa

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, D, G, J (A, D, G, J)	Glutamyl-tRNA(Gln) amidotransferase subunit A	polymer	484	51912.5	4	UniProt (Q9HVT8) Pfam (PF01425)	Pseudomonas aeruginosa PAO1	Glu-ADT subunit A, aspartyl-tRNA(Asn)/glutamyl-tRNA(Gln) amidotransferase subunit A
2	B, E, H, K (B, E, H, K)	Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B	polymer	481	51236.8	4	UniProt (Q9HVT7) Pfam (PF02934) Pfam (PF02637)	Pseudomonas aeruginosa PAO1	Asp/Glu-ADT subunit B, aspartyl-tRNA(Asn)/glutamyl-tRNA(Gln) amidotransferase subunit B
3	C, F, I, L (C, F, I, L)	Glutamyl-tRNA(Gln) amidotransferase subunit C	polymer	104	11558.0	4	UniProt (Q9HVT9) Pfam (PF02686)	Pseudomonas aeruginosa PAO1	Glu-ADT subunit C, aspartyl-tRNA(Asn)/glutamyl-tRNA(Gln) amidotransferase subunit C
4	M, N, O, P (M, N, O, P)	Aspartate--tRNA(Asp/Asn) ligase	polymer	599	67311.7	4	UniProt (Q51422) Pfam (PF01336) Pfam (PF00152) Pfam (PF02938)	Pseudomonas aeruginosa PAO1	Aspartyl-tRNA synthetase,AspRS,Non-discriminating aspartyl-tRNA synthetase,ND-AspRS
5	Q, R, S, T (Q, R, S, T)	76mer-tRNA	polymer	76	24471.5	4	GenBank (110227054)	Pseudomonas aeruginosa PAO1

Sequence modifications

B, E, H, K: 1 - 403 (UniProt: Q9HVT7)
B, E, H, K: 404 - 481 (PDB: 4WJ3)
C, F, I, L: 1 - 96 (UniProt: Q9HVT9)

PDB	External Database	Details
Met -7	-	expression tag
Gly -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag

M, N, O, P: 1 - 591 (UniProt: Q51422)

PDB	External Database	Details
Met -7	-	expression tag
Gly -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	20
	Total formula weight	825961.9
All*	Total formula weight	825961.9

*Water molecules are not included.