4WJ3

Crystal structure of the asparagine transamidosome from Pseudomonas aeruginosa

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000166	molecular_function	nucleotide binding
A	0003824	molecular_function	catalytic activity
A	0005524	molecular_function	ATP binding
A	0006412	biological_process	translation
A	0016874	molecular_function	ligase activity
A	0030956	cellular_component	glutamyl-tRNA(Gln) amidotransferase complex
A	0050567	molecular_function	glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity
B	0003824	molecular_function	catalytic activity
B	0016874	molecular_function	ligase activity
B	0016884	molecular_function	carbon-nitrogen ligase activity, with glutamine as amido-N-donor
C	0000166	molecular_function	nucleotide binding
C	0005524	molecular_function	ATP binding
C	0006412	biological_process	translation
C	0006450	biological_process	regulation of translational fidelity
C	0016874	molecular_function	ligase activity
C	0050566	molecular_function	asparaginyl-tRNA synthase (glutamine-hydrolyzing) activity
C	0050567	molecular_function	glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity
C	0070681	biological_process	glutaminyl-tRNAGln biosynthesis via transamidation
D	0000166	molecular_function	nucleotide binding
D	0003824	molecular_function	catalytic activity
D	0005524	molecular_function	ATP binding
D	0006412	biological_process	translation
D	0016874	molecular_function	ligase activity
D	0030956	cellular_component	glutamyl-tRNA(Gln) amidotransferase complex
D	0050567	molecular_function	glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity
E	0003824	molecular_function	catalytic activity
E	0016874	molecular_function	ligase activity
E	0016884	molecular_function	carbon-nitrogen ligase activity, with glutamine as amido-N-donor
F	0000166	molecular_function	nucleotide binding
F	0005524	molecular_function	ATP binding
F	0006412	biological_process	translation
F	0006450	biological_process	regulation of translational fidelity
F	0016874	molecular_function	ligase activity
F	0050566	molecular_function	asparaginyl-tRNA synthase (glutamine-hydrolyzing) activity
F	0050567	molecular_function	glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity
F	0070681	biological_process	glutaminyl-tRNAGln biosynthesis via transamidation
G	0000166	molecular_function	nucleotide binding
G	0003824	molecular_function	catalytic activity
G	0005524	molecular_function	ATP binding
G	0006412	biological_process	translation
G	0016874	molecular_function	ligase activity
G	0030956	cellular_component	glutamyl-tRNA(Gln) amidotransferase complex
G	0050567	molecular_function	glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity
H	0003824	molecular_function	catalytic activity
H	0016874	molecular_function	ligase activity
H	0016884	molecular_function	carbon-nitrogen ligase activity, with glutamine as amido-N-donor
I	0000166	molecular_function	nucleotide binding
I	0005524	molecular_function	ATP binding
I	0006412	biological_process	translation
I	0006450	biological_process	regulation of translational fidelity
I	0016874	molecular_function	ligase activity
I	0050566	molecular_function	asparaginyl-tRNA synthase (glutamine-hydrolyzing) activity
I	0050567	molecular_function	glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity
I	0070681	biological_process	glutaminyl-tRNAGln biosynthesis via transamidation
J	0000166	molecular_function	nucleotide binding
J	0003824	molecular_function	catalytic activity
J	0005524	molecular_function	ATP binding
J	0006412	biological_process	translation
J	0016874	molecular_function	ligase activity
J	0030956	cellular_component	glutamyl-tRNA(Gln) amidotransferase complex
J	0050567	molecular_function	glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity
K	0003824	molecular_function	catalytic activity
K	0016874	molecular_function	ligase activity
K	0016884	molecular_function	carbon-nitrogen ligase activity, with glutamine as amido-N-donor
L	0000166	molecular_function	nucleotide binding
L	0005524	molecular_function	ATP binding
L	0006412	biological_process	translation
L	0006450	biological_process	regulation of translational fidelity
L	0016874	molecular_function	ligase activity
L	0050566	molecular_function	asparaginyl-tRNA synthase (glutamine-hydrolyzing) activity
L	0050567	molecular_function	glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity
L	0070681	biological_process	glutaminyl-tRNAGln biosynthesis via transamidation
M	0000166	molecular_function	nucleotide binding
M	0003676	molecular_function	nucleic acid binding
M	0004812	molecular_function	aminoacyl-tRNA ligase activity
M	0004815	molecular_function	aspartate-tRNA ligase activity
M	0005524	molecular_function	ATP binding
M	0005737	cellular_component	cytoplasm
M	0006412	biological_process	translation
M	0006418	biological_process	tRNA aminoacylation for protein translation
M	0006422	biological_process	aspartyl-tRNA aminoacylation
M	0016874	molecular_function	ligase activity
M	0050560	molecular_function	aspartate-tRNA(Asn) ligase activity
N	0000166	molecular_function	nucleotide binding
N	0003676	molecular_function	nucleic acid binding
N	0004812	molecular_function	aminoacyl-tRNA ligase activity
N	0004815	molecular_function	aspartate-tRNA ligase activity
N	0005524	molecular_function	ATP binding
N	0005737	cellular_component	cytoplasm
N	0006412	biological_process	translation
N	0006418	biological_process	tRNA aminoacylation for protein translation
N	0006422	biological_process	aspartyl-tRNA aminoacylation
N	0016874	molecular_function	ligase activity
N	0050560	molecular_function	aspartate-tRNA(Asn) ligase activity
O	0000166	molecular_function	nucleotide binding
O	0003676	molecular_function	nucleic acid binding
O	0004812	molecular_function	aminoacyl-tRNA ligase activity
O	0004815	molecular_function	aspartate-tRNA ligase activity
O	0005524	molecular_function	ATP binding
O	0005737	cellular_component	cytoplasm
O	0006412	biological_process	translation
O	0006418	biological_process	tRNA aminoacylation for protein translation
O	0006422	biological_process	aspartyl-tRNA aminoacylation
O	0016874	molecular_function	ligase activity
O	0050560	molecular_function	aspartate-tRNA(Asn) ligase activity
P	0000166	molecular_function	nucleotide binding
P	0003676	molecular_function	nucleic acid binding
P	0004812	molecular_function	aminoacyl-tRNA ligase activity
P	0004815	molecular_function	aspartate-tRNA ligase activity
P	0005524	molecular_function	ATP binding
P	0005737	cellular_component	cytoplasm
P	0006412	biological_process	translation
P	0006418	biological_process	tRNA aminoacylation for protein translation
P	0006422	biological_process	aspartyl-tRNA aminoacylation
P	0016874	molecular_function	ligase activity
P	0050560	molecular_function	aspartate-tRNA(Asn) ligase activity

Functional Information from PROSITE/UniProt

site_id	PS00571
Number of Residues	32
Details	AMIDASES Amidases signature. GGSSGGsAAaVAarllpaAtGtDtGgSIRqPA

Chain	Residue	Details
A	GLY150-ALA181

---

site_id	PS01234
Number of Residues	15
Details	GATB Glutamyl-tRNA(Gln) amidotransferase subunit B signature. LNRagtPLLEIvSeP

Chain	Residue	Details
B	LEU144-PRO158

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	8
Details	Active site: {"description":"Charge relay system","evidences":[{"source":"HAMAP-Rule","id":"MF_00120","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	4
Details	Active site: {"description":"Acyl-ester intermediate","evidences":[{"source":"HAMAP-Rule","id":"MF_00120","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	12
Details	Region: {"description":"Aspartate","evidences":[{"source":"HAMAP-Rule","id":"MF_00044","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	44
Details	Binding site: {"evidences":[{"source":"HAMAP-Rule","id":"MF_00044","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	8
Details	Site: {"description":"Important for tRNA non-discrimination"}

Chain

Residue

Details

---