4WA6

Structure of yeast SAGA DUBm with Sgf73 N59D mutant at 2.36 angstroms resolution

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, D	Ubiquitin carboxyl-terminal hydrolase 8	polymer	476	54033.6	2	UniProt (P50102) Pfam (PF02148) Pfam (PF00443) In PDB	Saccharomyces cerevisiae (Baker's yeast)	Deubiquitinating enzyme 8,Ubiquitin thioesterase 8,Ubiquitin-specific-processing protease 8
2	B, F	Transcription and mRNA export factor SUS1	polymer	96	11094.5	2	UniProt (Q6WNK7) Pfam (PF10163) In PDB	Saccharomyces cerevisiae (Baker's yeast)
3	C, G	SAGA-associated factor 11	polymer	99	11297.6	2	UniProt (A6ZWK1) Pfam (PF18519) Pfam (PF08209) In PDB	Saccharomyces cerevisiae (Baker's yeast)
4	E, H	SAGA-associated factor 73	polymer	96	10825.3	2	UniProt (P53165) Pfam (PF18508) In PDB	Saccharomyces cerevisiae (Baker's yeast)	73 kDa SAGA-associated factor,SAGA histone acetyltransferase complex 73 kDa subunit
5	A, C, E, D, G...	ZINC ION	non-polymer		65.4	15	Chemie (ZN)
6		water	water		18.0	139	Chemie (HOH)

Sequence modifications

A, D: 1 - 471 (UniProt: P50102)

PDB	External Database	Details
Gly -4	-	expression tag
Ala -3	-	expression tag
Ala -2	-	expression tag
Ala -1	-	expression tag
Ala 0	-	expression tag

E, H: 1 - 96 (UniProt: P53165)

PDB	External Database	Details
Asp 59	Asn 59	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	174502.0
Non-Polymers*	Number of molecules	15
	Total formula weight	981.1
All*	Total formula weight	175483.2

*Water molecules are not included.