4WA6
Structure of yeast SAGA DUBm with Sgf73 N59D mutant at 2.36 angstroms resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-06-28 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.034 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 81.076, 68.004, 137.858 |
Unit cell angles | 90.00, 106.75, 90.00 |
Refinement procedure
Resolution | 47.410 - 2.360 |
R-factor | 0.18615 |
Rwork | 0.183 |
R-free | 0.24380 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.014 |
RMSD bond angle | 1.654 |
Data scaling software | HKL-2000 |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 47.410 |
High resolution limit [Å] | 2.360 |
Number of reflections | 59279 |
<I/σ(I)> | 23 |
Completeness [%] | 100.0 |
Redundancy | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 100mM Bis Tris, 18% PEG3350, 100mM Ammonium Sulfate |