4W5V

Crystal structure of Human SUMO E2-conjugating enzyme (Ubc9) in complex with E1-activating enzyme (Uba2) ubiquitin fold domain (Ufd)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	SUMO-conjugating enzyme UBC9	polymer	163	18442.3	1	UniProt (P63279) Pfam (PF00179)	Homo sapiens (Human)	SUMO-protein ligase,Ubiquitin carrier protein 9,Ubiquitin carrier protein I,Ubiquitin-conjugating enzyme E2 I,Ubiquitin-protein ligase I,p18
2	B (B)	SUMO-activating enzyme subunit 2	polymer	122	13358.0	1	UniProt (Q9UBT2) Pfam (PF14732)	Homo sapiens (Human)	Anthracycline-associated resistance ARX,Ubiquitin-like 1-activating enzyme E1B,Ubiquitin-like modifier-activating enzyme 2
3	C (A)	FORMIC ACID	non-polymer		46.0	1	Chemie (FMT)
4	D (B)	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
5	E (B)	POTASSIUM ION	non-polymer		39.1	1	Chemie (K)
6	F, G (A, B)	water	water		18.0	74	Chemie (HOH)

Sequence modifications

A: 1 - 158 (UniProt: P63279)

PDB	External Database	Details
Gly -4	-	expression tag
Pro -3	-	expression tag
Leu -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag

B: 445 - 561 (UniProt: Q9UBT2)

PDB	External Database	Details
Gly 440	-	expression tag
Pro 441	-	expression tag
Leu 442	-	expression tag
Gly 443	-	expression tag
Ser 444	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	31800.2
Non-Polymers*	Number of molecules	3
	Total formula weight	177.2
All*	Total formula weight	31977.5

*Water molecules are not included.